In this study, An AHK4 CHASE domain was used to construct an electrochemical cytokinin biosensor using ferrocene as the electrochemical mediator. Upon addition of cytokinin, the binding of cytokinin and AHK4 led to dimerization, which blocked electron transfer between ferrocene and the electrode, and the redox peak current of ferrocene was gradually reduced. Cytokinin was detected by recording the change of the ferrocene redox peak current. The biosensor shows a linear range of 50–400 nM with a linear regression equation of i_p = 0.0086c + 0.732 (R² = 0.993) with i_p in μA and c in nM and a detection limit (LOD) of 1.5 nM (S/N = 3). The biosensor exhibits excellent performance that avoids interference of other types of plant hormones and was successfully applied to the detection of cytokinins in bean sprouts.

在这项研究中，AHK4 CHASE 域用于构建电化学细胞分裂素生物传感器，使用二茂铁作为电化学介质。添加细胞分裂素后，细胞分裂素与AHK4结合导致二聚化，阻断二茂铁与电极之间的电子传递，二茂铁的氧化还原峰值电流逐渐降低。通过记录二茂铁氧化还原峰值电流的变化来检测细胞分裂素。生物传感器显示线性范围为 50–400 nM，线性回归方程为i _p  = 0.0086 c  + 0.732 (R ²  = 0.993)，其中i _p为 μA 和c以 nM 为单位，检测限 (LOD) 为 1.5 nM (S/N = 3)。该生物传感器表现出优异的性能，避免了其他类型植物激素的干扰，并成功应用于豆芽中细胞分裂素的检测。