The combined effects of ethylenediaminetetraacetic acid (EDTA) and bacteriophage (phage) treatment of foodborne pathogens were investigated. Although viable counts for Campylobacter jejuni decreased by 1.5 log after incubation for 8 h in the presence of phage PC10, re-growth was observed thereafter. The combination of phage PC10 and 1 mM EDTA significantly inhibited the re-growth of C. jejuni. The viable counts for C. jejuni decreased by 2.6 log (P < 0.05) compared with that of the initial count after 24 h. Moreover, EDTA at 0.67 or 1.3 mM, combined with the specific lytic phages, also effectively inhibited the re-growth of phage-resistant cells of Campylobacter coli, Salmonella enterica serovar Enteritidis, and Salmonella enterica serovar Typhimurium. In addition, the combined effects of lytic phages and EDTA were investigated on the viability of Campylobacter in BHI broth at low temperatures followed by the optimum growth temperature. The re-growth of C. coli was significantly inhibited by the coexistence of 1.3 mM EDTA, and the viable counts of surviving bacteria was about the same as the initial viable count after the incubation. This is the first study demonstrating the combined use of lytic phages and EDTA is effective in inhibiting the re-growth of phage-resistant bacteria in Gram-negative bacteria.

研究了乙二胺四乙酸 (EDTA) 和噬菌体 (phage) 处理食源性病原体的联合作用。尽管在噬菌体 PC10 存在下孵育 8 小时后空肠弯曲杆菌的活菌数减少了 1.5 log，但此后观察到了重新生长。噬菌体 PC10 和 1 mM EDTA 的组合显着抑制了空肠弯曲杆菌的再生长。 与 24 小时后的初始计数相比，空肠弯曲杆菌的活菌数减少了 2.6 log ( P < 0.05)。此外，0.67 或 1.3 mM EDTA 与特异性裂解噬菌体结合，也有效抑制了弯曲杆菌、肠炎沙门氏菌血清型的噬菌体抗性细胞的再生长，和沙门氏菌肠炎鼠伤寒沙门氏菌。此外，研究了裂解噬菌体和 EDTA 的联合作用对低温下 BHI 肉汤中弯曲杆菌的生存能力，然后是最佳生长温度。的再生长C.大肠杆菌1.3毫摩尔EDTA的共存被显著抑制，存活的细菌的活菌数为约相同温育后的初始活菌计数。这是第一项证明裂解噬菌体和 EDTA 联合使用可有效抑制革兰氏阴性菌中噬菌体抗性细菌的再生长的研究。