Defatted Schizochytrium residue was used as a supplementary feedstock for efficiency erythritol production in this study. Microalgae are attractive feedstocks for the microbial process. However, proper enzymatic hydrolysis and toxicity alleviation must be considered for efficient fermentation. A strategy was proposed herein for producing erythritol from enzymatic hydrolysate of the defatted Schizochytrium residue (SRH), resorting to the wheat (Triticum aestivum L.) straw biochar-incorporated solid-state fermentation (SSF) of agricultural wastes to relieve the toxicant inhibition. Enhanced erythritol production (190.5 mg/gds) was achieved in batch SSF supplemented with 4% biochar and 20 % concentrated SRH, which was obtained from the cellulose–protease (1:1) system performed under pH 5.0 and 45 °C for 24 h with 0.8 % (m/v) of enzyme addition. Fed- and repeated-batch fermentation demonstrated enduring high cell viability and improved erythritol yield of 223.2 and 205.4 mg/gds, respectively. Cost analysis suggested high availabilities of raw substrates for erythritol cultivation, which was energy conserving and operable. Moreover, SRH was proven to be an economic alternative feedstock for low-cost erythritol production via the biochar-incorporated SSF.

在本研究中，脱脂裂殖壶菌残渣用作高效赤藓糖醇生产的补充原料。微藻是微生物过程的有吸引力的原料。然而，有效发酵必须考虑适当的酶水解和毒性减轻。本文提出了一种从脱脂裂殖壶菌残渣 (SRH) 的酶促水解产物生产赤藓糖醇的策略，采用小麦 ( Triticum aestivumL.) 结合秸秆生物炭的农业废物固态发酵 (SSF)，以减轻毒物抑制。在添加 4% 生物炭和 20% 浓缩 SRH 的批次 SSF 中实现了赤藓糖醇产量的提高（190.5 mg/gds），这是从纤维素-蛋白酶 (1:1) 系统中获得的，在 pH 5.0 和 45 °C 下进行 24 小时添加 0.8 % (m/v) 的酶。补料和重复分批发酵显示出持久的高细胞活力和提高的赤藓糖醇产量，分别为 223.2 和 205.4 mg/gds。成本分析表明，赤藓糖醇培养的原始底物具有很高的可用性，这是节能和可操作的。此外，SRH 被证明是一种经济的替代原料，可通过掺入生物炭的 SSF 进行低成本赤藓糖醇生产。