Abstract—

Slow-growing, hard-to-cultivate prokaryotes require special conditions for laboratory cultivation. The difficulty in cultivation may often prevent depositing the new isolates in the culture collections in order to validate their taxonomic status. The sulfate-reducing firmicute “Desulforudis audaxviator” strain BYF, has been isolated from a deep thermal borehole in West Siberia. In this study we showed that decreasing the concentrations of calcium and phosphate, as well as addition of a polyamine spermidine, increased the growth rate and biomass formation by these bacterium.

中文翻译：

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一种用于培养“脱硫菌”的新型培养基

摘要——

生长缓慢、难以培养的原核生物需要特殊的实验室培养条件。培养中的困难通常会阻止将新分离株存放在培养物保藏中心以验证其分类状态。硫酸盐还原型厚壁菌“ Desulforudis audaxviator ”菌株 BYF 已从西西伯利亚的一个深热钻孔中分离出来。在这项研究中，我们表明降低钙和磷酸盐的浓度，以及添加多胺亚精胺，增加了这些细菌的生长速度和生物量形成。