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Probiotic Attributes and Inhibitory Effects of Lactobacillus plantarum MYS84 against the Growth and Biofilm Formation of Pseudomonas aeruginosa
Microbiology ( IF 1.5 ) Pub Date : 2021-06-08 , DOI: 10.1134/s0026261721030103
Poornachandra Rao K , Hemanth Kumar N , Rakesh Somashekaraiah , M. Murali , Shobha J , M. Y. Sreenivasa

Abstract

Manufacture of fermented foods/products, which, apart from basic nutrition, have health-promoting effects, is flourishing. Within the field of fermented foods, rapidly expanding is the area of probiotics. Probiotic bacteria are gaining growing attention in the last two decades as a result of the constantly increasing scientific evidence of their beneficial effects on human health. In the current study, the isolate Lactobacillus plantarum MYS84 was identified by morphological, biochemical, physiological, and genetic methods. The standard in vitro techniques revealed that the isolated strain possessed good probiotic attributes. The cell-free supernatant (CFS) was active against Pseudomonas aeruginosa MTCC 7903, probably due to its proteinaceous nature, and it retained activity after treatment with catalase, lysozyme, and papain. It was also heat-resistant and acid-stable. Moreover, the CFS exhibited strong biofilm inhibition (78%).



中文翻译:

植物乳杆菌 MYS84 对铜绿假单胞菌生长和生物膜形成的益生菌特性和抑制作用

摘要

除了基本营养外,还具有促进健康的作用的发酵食品/产品的制造正在蓬勃发展。在发酵食品领域,快速扩张的是益生菌领域。由于越来越多的科学证据表明益生菌对人类健康有益,因此在过去的二十年里,益生菌越来越受到关注。在目前的研究中,分离株植物乳杆菌MYS84 是通过形态学、生化、生理和遗传方法鉴定的。标准的体外技术表明,分离的菌株具有良好的益生菌特性。无细胞上清液 (CFS) 对铜绿假单胞菌有活性MTCC 7903,可能是由于其蛋白质性质,在用过氧化氢酶、溶菌酶和木瓜蛋白酶处理后仍保持活性。它还具有耐热性和酸稳定性。此外,CFS 表现出强烈的生物膜抑制(78%)。

更新日期:2021-06-08
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