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Radiosensitivity of radiotherapy patients: The effect of individual DNA repair capacity
Mutation Research/Genetic Toxicology and Environmental Mutagenesis ( IF 1.9 ) Pub Date : 2021-06-07 , DOI: 10.1016/j.mrgentox.2021.503371
Eliana E Ocolotobiche 1 , Ricard Marcos Dauder 2 , Alba Mabel Güerci 1
Affiliation  

Individual radiosensitivity is a critical problem in radiotherapy because of the treatment restrictions it imposes. We have tested whether induction/repair of genomic lesions correlates with the acute cutaneous effects of radiotherapy. Peripheral blood samples of 56 healthy volunteers and 18 patients with breast cancer were studied. DNA damage and DNA repair capacity were assessed in vitro (alkaline comet assay). Patients without skin reaction did not show significant differences from healthy individuals, with respect to either initial or radiation-induced DNA damage. Similar DNA repair kinetics, fitting a decreasing exponential response, were observed in both groups, and there were no significant differences in residual genotoxic damage. In contrast, patients exhibiting acute side effects showed significantly lower DNA repair ability and significantly more residual damage, compared to patients without radiotoxicity. This approach may help to identify patients who are at greater risk of radiotherapy side effects. However, many other factors, such as dosimetry, irradiated volume, and lifestyle should also be considered in the evaluation of individual radiosensitivity.



中文翻译:

放疗患者的放射敏感性:个体DNA修复能力的影响

由于其强加的治疗限制,个体放射敏感性是放射治疗中的一个关键问题。我们已经测试了基因组病变的诱导/修复是否与放疗的急性皮肤效应相关。研究了 56 名健康志愿者和 18 名乳腺癌患者的外周血样本。体外评估 DNA 损伤和 DNA 修复能力(碱性彗星试验)。没有皮肤反应的患者与健康个体在初始或辐射诱导的 DNA 损伤方面没有显着差异。在两组中都观察到类似的 DNA 修复动力学,符合递减的指数反应,并且在残留的遗传毒性损伤方面没有显着差异。相比之下,与没有放射毒性的患者相比,表现出急性副作用的患者的 DNA 修复能力显着降低,残留损伤显着更多。这种方法可能有助于识别放疗副作用风险更大的患者。然而,在评估个体放射敏感性时还应考虑许多其他因素,例如剂量测定、照射量和生活方式。

更新日期:2021-06-08
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