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Rapid Cell Transfer by Means of Nylon Mesh to Improve Cellular Diagnosis: The Role of Immunocytochemistry
Acta Cytologica ( IF 1.8 ) Pub Date : 2021-06-07 , DOI: 10.1159/000516555 Satoshi Morito 1 , Takao Nitanda 2 , Ryuko Tsukamoto 2 , Shingo Kamoshida 3 , Hiroshi Yasui 1 , Tomoo Itoh 2 , Hiroyuki Ohsaki 3
Acta Cytologica ( IF 1.8 ) Pub Date : 2021-06-07 , DOI: 10.1159/000516555 Satoshi Morito 1 , Takao Nitanda 2 , Ryuko Tsukamoto 2 , Shingo Kamoshida 3 , Hiroshi Yasui 1 , Tomoo Itoh 2 , Hiroyuki Ohsaki 3
Affiliation
Immunocytochemistry (ICC) is an important ancillary technique in clinical cytology for not only identifying and characterizing tumor cells but also gaining prognostic or therapeutic information. Although cell blocks are often prepared for immunocytochemical evaluation of body cavity fluid and fine-needle aspiration specimens, they are not suitable for hypocellular samples. Liquid-based cytology can help prepare additional smears from residual cytological specimens. However, since conventional methods are used for nongynecological specimens in most laboratories, ICC is often limited by the number of cytological smears. Cell transfer methods permit to evaluate several immunocytochemical markers in a single cytological smear. Yet, these methods have some limitations; for example, they are time-consuming (about 3–40 h) and medium membranes with their attached cells are occasionally stretched or torn when peeled off the slides. Therefore, in an attempt to solve these problems, we developed a rapid and reliable cell transfer method using a nylon mesh. Our method requires no special equipment or reagent and can significantly reduce the turnaround time, as compared to previous methods.
Acta Cytologica
中文翻译:
通过尼龙网快速转移细胞以改善细胞诊断:免疫细胞化学的作用
免疫细胞化学 (ICC) 是临床细胞学中的一项重要辅助技术,不仅可以识别和表征肿瘤细胞,还可以获取预后或治疗信息。虽然细胞块通常用于体腔液和细针穿刺标本的免疫细胞化学评估,但它们不适合细胞不足的样品。液基细胞学可以帮助从残留的细胞学标本中制备额外的涂片。然而,由于大多数实验室对非妇科标本使用常规方法,因此 ICC 通常受到细胞学涂片数量的限制。细胞转移方法允许在单个细胞学涂片中评估几种免疫细胞化学标记。然而,这些方法有一些局限性;例如,它们很耗时(大约 3-40 小时),并且当从载玻片上剥离时,带有附着细胞的中膜偶尔会被拉伸或撕裂。因此,为了解决这些问题,我们开发了一种使用尼龙网的快速可靠的细胞转移方法。与以前的方法相比,我们的方法不需要特殊设备或试剂,可以显着减少周转时间。
细胞学学报
更新日期:2021-06-07
Acta Cytologica
中文翻译:
通过尼龙网快速转移细胞以改善细胞诊断:免疫细胞化学的作用
免疫细胞化学 (ICC) 是临床细胞学中的一项重要辅助技术,不仅可以识别和表征肿瘤细胞,还可以获取预后或治疗信息。虽然细胞块通常用于体腔液和细针穿刺标本的免疫细胞化学评估,但它们不适合细胞不足的样品。液基细胞学可以帮助从残留的细胞学标本中制备额外的涂片。然而,由于大多数实验室对非妇科标本使用常规方法,因此 ICC 通常受到细胞学涂片数量的限制。细胞转移方法允许在单个细胞学涂片中评估几种免疫细胞化学标记。然而,这些方法有一些局限性;例如,它们很耗时(大约 3-40 小时),并且当从载玻片上剥离时,带有附着细胞的中膜偶尔会被拉伸或撕裂。因此,为了解决这些问题,我们开发了一种使用尼龙网的快速可靠的细胞转移方法。与以前的方法相比,我们的方法不需要特殊设备或试剂,可以显着减少周转时间。
细胞学学报