Hydrogen peroxide is an important factor involved in the senescence and quality deterioration of postharvest fruit. However, few studies have examined hydrogen peroxide sensors and their expression patterns in grape. Here, postharvest grape berries were treated with H₂O₂ and the ROS inhibitor hypotaurine (HT). HT reduced the H₂O₂ content by increasing ROS-scavenging enzyme activity, whereas H₂O₂ accelerated senescence. Four hydrogen peroxide sensors (VvHPCA1, 2, 3, and 4) were identified and isolated from the grape genome. Sequence analysis indicated that VvHPCAs shared a conserved structure, including the LRR-RK and hydrogen peroxide (HP) domains, with other homologous hydrogen peroxide sensor genes. Furthermore, the expression patterns of the four VvHPCAs were investigated under H₂O₂ and HT treatment in grape. The expression levels of the four VvHPCAs were lower in grape berries treated with HT than in grape berries treated with H₂O₂ or water treatment (Control) in at least one of the sampling points during storage. VvHPCAs are cytomembrane proteins with stress-responsive cis-acting elements in their promoters, indicating that these four identified VvHPCAs might function as hydrogen peroxide sensors in grape and regulate hydrogen peroxide signaling and metabolism. Thus, VvHPCAs are capable of responding to postharvest ROS stress. These findings provided new insight into the function of these four VvHPCAs, which are potential candidate genes involved in regulating ROS stress in grape.

过氧化氢是影响采后果实衰老和品质劣化的重要因素。然而，很少有研究检查过氧化氢传感器及其在葡萄中的表达模式。在这里，收获后的葡萄浆果用 H ₂ O ₂和 ROS 抑制剂亚牛磺酸 (HT) 处理。HT通过增加ROS清除酶活性降低H ₂ O ₂含量，而H ₂ O ₂加速衰老。四个过氧化氢传感器（VvHPCA1，2，3，和4）被确定和从葡萄基因组中分离。序列分析表明VvHPCAs与其他同源过氧化氢传感器基因共享一个保守结构，包括 LRR-RK 和过氧化氢 (HP) 结构域。此外，研究了四种VvHPCA在 H ₂ O ₂和 HT 处理下在葡萄中的表达模式。HT处理的葡萄浆果中4种VvHPCA的表达水平低于H ₂ O ₂处理的葡萄浆果或在储存期间至少在一个采样点进行水处理（控制）。VvHPCAs 是细胞膜蛋白，其启动子中具有应激反应顺式作用元件，表明这四种已鉴定的 VvHPCAs 可能在葡萄中充当过氧化氢传感器并调节过氧化氢信号传导和代谢。因此，VvHPCAs 能够应对采后 ROS 应激。这些发现为这四种 VvHPCA 的功能提供了新的见解，它们是参与调节葡萄 ROS 胁迫的潜在候选基因。