Purpose

We evaluated the role of water levels and plant-associated effects on the composition of bacterial communities and the soil organic matter (SOM) composition in wetland ecosystem.

Materials and methods

Soil (0–15 cm) associated with Phragmites communis, Triarrhena lutarioriparia, Carex cinerascens, and Zizania latifolia was sampled in different water levels (as proxied by sampling time) to analyze soil SOM composition via diffuse reflectance infrared Fourier transform spectroscopy in the mid-infrared range analysis and to analyze soil bacterial communities by high-throughput 16S rRNA gene sequencing.

Results and discussion

Soil dominant SOM composition (1623–1635 cm⁻¹ and 3444–2697 cm⁻¹) were obviously influenced by water levels not plant species (p < 0.05). Proteobacteria, Chloroflexi, Acidobacteria, and Firmicutes were the most abundant phyla and exhibited significantly different distributions across the soils with Z. latifolia, P. communis, T. lutarioriparia, and C. cinerascens communities and the non-plant flat mud soil (p < 0.05). Redundancy analysis and variation partitioning analysis indicated that the Wc, active organic carbon, TC_stem, Tem_soil, and water level significantly influenced the dominant soil bacterial taxonomic composition, while the soil bacteria may influence the SOM composition.

Conclusions

These findings suggested that soil SOM composition and bacterial communities were strongly affected by plant species and water levels. This study provides an in-depth insight into how specific SOM components related to the different distributions of the specific bacterial taxonomic groups that are responsible for key ecological functions in wetland ecosystem soils.

中文翻译：

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鄱阳湖优势植物和水文波动对土壤有机质组成与土壤细菌群落结构的影响

目的

我们评估了水位和植物相关效应对湿地生态系统中细菌群落组成和土壤有机质 (SOM) 组成的作用。

材料和方法

在不同水位（以采样时间为代表）对与芦苇、Triarrhena lutarioriparia、Carex cinerascens和Zizania latifolia相关的土壤（0-15 cm）进行采样，以通过漫反射红外傅立叶变换光谱分析土壤 SOM 组成。红外范围分析和通过高通量 16S rRNA 基因测序分析土壤细菌群落。

结果和讨论

土壤占主导地位的 SOM 组成（1623-1635 cm ^-1和 3444-2697 cm ^-1）明显受水位影响，而非植物物种（p  < 0.05）。变形菌，绿弯菌门，酸杆菌门和厚壁菌门是最丰富的门类并表现出在整个土壤显著不同分布与菰，芦苇，T. lutarioriparia，和C. cinerascens社区和非植物平坦泥土（p  < 0.05）。冗余分析和变异划分分析表明，Wc、活性有机碳、TC_茎、Tem_土壤和水位显着影响主要土壤细菌分类组成，而土壤细菌可能影响 SOM 组成。

结论

这些发现表明土壤 SOM 组成和细菌群落受植物物种和水位的强烈影响。这项研究深入了解了特定 SOM 成分如何与负责湿地生态系统土壤中关键生态功能的特定细菌分类群的不同分布相关。