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The tissue expression of MCT3, MCT8, and MCT9 genes in women with breast cancer
Genes & Genomics ( IF 2.1 ) Pub Date : 2021-06-07 , DOI: 10.1007/s13258-021-01116-w
Ehsan Sohrabi 1 , Masoumeh Moslemi 1 , Ehsan Rezaie 2 , Nahid Nafissi 3 , Mansoor Khaledi 4 , Hamed Afkhami 4 , Javad Fathi 5 , Ali Zekri 1
Affiliation  

Background

Breast cancer (BC) is a common malignancy with a high mortality rate. Malignant cell transformation is associated with metabolic changes. One group of proteins that are affected is the monocarboxylate transporters (MCTs-SLC16A). The MCTs comprise 14 members, and they play an important role in the growth, proliferation, and metabolism of cancer cells by transporting monocarboxylates such as lactate, pyruvate and thyroid hormones.

Objective

We aimed to evaluate the expression of MCT3 (SLC16A8), MCT8 (SLC16A2) and MCT9 (SLC16A9) genes in breast cancer samples, comparing to normal adjacent tissues.

Methods

Forty paired breast cancer tumor samples, the adjacent non-tumor and five healthy tissues were collected. Three cancer cell lines (MCF-7, MDA-MB-231, and SKBR3) were also analyzed. The expression of SLC16A8, SLC16A2 and SLC16A9 were assessed using quantitative real-time PCR. The relationship between gene expression with the pathological features of the tumors, and the hormone receptors status of the patient’s tumors were also analyzed.

Results

There was a significantly lower expression of the MCT3 gene in tumor samples compared to adjacent normal tissue and healthy samples (p value < 0.05). There was a significant difference in the expression of all three candidate genes between the BC tissues and normal tissues, and for the, tissues with different hormone receptor status and the molecular subtypes. Altered MCT8 and MCT9 gene expression was associated with a reduced survival

Conclusion

MCT3 expression is significantly downregulated in breast cancer tissue. MCT3 may represent a novel therapeutic target in breast cancer patients, or in some hormone receptor subgroups.



中文翻译:

MCT3、MCT8、MCT9基因在女性乳腺癌组织中的表达

背景

乳腺癌(BC)是一种常见的恶性肿瘤,死亡率很高。恶性细胞转化与代谢变化有关。受影响的一组蛋白质是单羧酸转运蛋白 (MCTs-SLC16A)。MCT由14个成员组成,它们通过转运乳酸、丙酮酸和甲状腺激素等单羧酸盐在癌细胞的生长、增殖和代谢中发挥重要作用。

客观的

我们的目的是评估乳腺癌样本中MCT3 (SLC16A8)、MCT8 (SLC16A2) 和MCT9 (SLC16A9) 基因的表达,并与正常邻近组织进行比较。

方法

收集了四十对乳腺癌肿瘤样本、相邻的非肿瘤组织和五个健康组织。还分析了三种癌细胞系(MCF-7、MDA-MB-231 和 SKBR3)。使用定量实时 PCR 评估 SLC16A8、SLC16A2 和 SLC16A9 的表达。还分析了基因表达与肿瘤病理特征、患者肿瘤激素受体状态的关系。

结果

与邻近的正常组织和健康样本相比,肿瘤样本中MCT3基因的表达显着降低(p 值 < 0.05)。BC组织和正常组织之间以及不同激素受体状态和分子亚型的组织之间所有三个候选基因的表达均存在显着差异。改变的 MCT8MCT9基因表达与存活率降低有关

结论

MCT3 表达在乳腺癌组织中显着下调。MCT3 可能代表乳腺癌患者或某些激素受体亚组的新治疗靶点。

更新日期:2021-06-07
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