In this paper, we developed a simply designed detection method for logic gates by using aptamers, cetyltrimethyl ammonium bromide, together with melamine and cyromazine to control the aggregation and dispersion of gold nanoparticles (AuNPs). First, either melamine or cyromazine can induce the aggregation of AuNPs, supporting an OR gate to detect whether they were present or not. Second, based on the fact that aptamer T31 can specifically bind with melamine, preventing the aggregation of AuNPs, an improved INHIBIT gate was also fabricated to find whether there was melamine. It has a detection limit of 0.12 ppm by the naked eye for the detection of melamine, and the limit of detection (LOD) by spectrophotometer is 2.2 ppb. Third, with the adsorption of aptamer Tcy1 on AuNPs and the strong coordination of Tcy1 with cyromazine, the addition of cyromazine and CTAB immediately resulted in the aggregation of AuNPs, giving rise to an AND gate. This gate has a detection limit of 0.17 ppm by the naked eye and the limit of detection (LOD) is 9.0 ppb by spectrophotometer. The system provided a good platform for the development of functional logic systems.

在本文中，我们开发了一种简单设计的逻辑门检测方法，通过使用适体、十六烷基三甲基溴化铵以及三聚氰胺和环丙胺来控制金纳米粒子 (AuNPs) 的聚集和分散。首先，三聚氰胺或环丙氨嗪可以诱导 AuNPs 的聚集，支持 OR 门来检测它们是否存在。其次，基于适体 T31 可以与三聚氰胺特异性结合，防止 AuNPs 聚集的事实，还制造了改进的 INHIBIT 门以检测是否存在三聚氰胺。它对三聚氰胺的检测肉眼检测限为0.12 ppm，分光光度计检测限（LOD）为2.2 ppb。第三，随着适配体Tcy1在AuNPs上的吸附以及Tcy1与环丙氨嗪的强配位，添加环丙氨嗪和 CTAB 会立即导致 AuNP 的聚集，从而产生与门。该门的肉眼检测限为 0.17 ppm，分光光度计检测限 (LOD) 为 9.0 ppb。该系统为功能逻辑系统的开发提供了良好的平台。