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Circulating immune cell populations related to primary breast cancer, surgical removal, and radiotherapy revealed by flow cytometry analysis
Breast Cancer Research ( IF 7.4 ) Pub Date : 2021-06-05 , DOI: 10.1186/s13058-021-01441-8
Sarah Cattin 1 , Benoît Fellay 2 , Antonello Calderoni 3 , Alexandre Christinat 3 , Laura Negretti 4 , Maira Biggiogero 4, 5 , Alberto Badellino 4, 5 , Anne-Lise Schneider 6 , Pelagia Tsoutsou 6, 7 , Alessandra Franzetti Pellanda 4, 5 , Curzio Rüegg 1
Affiliation  

Advanced breast cancer (BC) impact immune cells in the blood but whether such effects may reflect the presence of early BC and its therapeutic management remains elusive. To address this question, we used multiparametric flow cytometry to analyze circulating leukocytes in patients with early BC (n = 13) at the time of diagnosis, after surgery, and after adjuvant radiotherapy, compared to healthy individuals. Data were analyzed using a minimally supervised approach based on FlowSOM algorithm and validated manually. At the time of diagnosis, BC patients have an increased frequency of CD117+CD11b+ granulocytes, which was significantly reduced after tumor removal. Adjuvant radiotherapy increased the frequency of CD45RO+ memory CD4+ T cells and CD4+ regulatory T cells. FlowSOM algorithm analysis revealed several unanticipated populations, including cells negative for all markers tested, CD11b+CD15low, CD3+CD4−CD8−, CD3+CD4+CD8+, and CD3+CD8+CD127+CD45RO+ cells, associated with BC or radiotherapy. This study revealed changes in blood leukocytes associated with primary BC, surgical removal, and adjuvant radiotherapy. Specifically, it identified increased levels of CD117+ granulocytes, memory, and regulatory CD4+ T cells as potential biomarkers of BC and radiotherapy, respectively. Importantly, the study demonstrates the value of unsupervised analysis of complex flow cytometry data to unravel new cell populations of potential clinical relevance.

中文翻译:

流式细胞术分析揭示与原发性乳腺癌、手术切除和放疗相关的循环免疫细胞群

晚期乳腺癌 (BC) 会影响血液中的免疫细胞,但这种影响是否反映了早期 BC 的存在及其治疗管理仍然难以捉摸。为了解决这个问题,我们使用多参数流式细胞术来分析早期 BC 患者(n = 13)在诊断时、手术后和辅助放疗后与健康个体相比的循环白细胞。使用基于 FlowSOM 算法的最小监督方法分析数据并手动验证。在诊断时,BC 患者 CD117+CD11b+ 粒细胞的频率增加,在肿瘤切除后显着减少。辅助放疗增加了 CD45RO+ 记忆 CD4+ T 细胞和 CD4+ 调节性 T 细胞的频率。FlowSOM 算法分析揭示了几个意料之外的群体,包括所有检测的标志物、CD11b+CD15low、CD3+CD4-CD8-、CD3+CD4+CD8+ 和 CD3+CD8+CD127+CD45RO+ 细胞均为阴性的细胞,与 BC 或放疗相关。该研究揭示了与原发性 BC、手术切除和辅助放疗相关的血液白细胞变化。具体而言,它确定了 CD117+ 粒细胞、记忆和调节性 CD4+ T 细胞水平升高,分别作为 BC 和放疗的潜在生物标志物。重要的是,该研究证明了对复杂流式细胞术数据进行无监督分析对揭示具有潜在临床相关性的新细胞群的价值。和辅助放疗。具体而言,它确定了 CD117+ 粒细胞、记忆和调节性 CD4+ T 细胞水平升高,分别作为 BC 和放疗的潜在生物标志物。重要的是,该研究证明了对复杂流式细胞术数据进行无监督分析对揭示具有潜在临床相关性的新细胞群的价值。和辅助放疗。具体而言,它确定了 CD117+ 粒细胞、记忆和调节性 CD4+ T 细胞水平升高,分别作为 BC 和放疗的潜在生物标志物。重要的是,该研究证明了对复杂流式细胞术数据进行无监督分析对揭示具有潜在临床相关性的新细胞群的价值。
更新日期:2021-06-07
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