Adriamycin (ADR) is an anticancer drug with important adverse effects, one of the most important of which is nephropathy. Plantago major (PM) is a widely used medicinal plant with many pharmacologic properties. The aim of the present study was to investigate the possible renoprotective effect of PM extract on ADR-induced renal fibrosis in rats. thirty-two male rats were divided into 4 groups: Control, ADR (5 mg/kg, iv), PM 600 + ADR, and PM 1200 + ADR. ADR was injected on the 7th day of the study. At the end of the 35th day of the experiment, left kidneys and serum samples were collected. The expression of collagen IV and transforming growth factor (TGF)-β were examined by immunohistochemistry. Interstitial fibrosis was estimated using Masson's trichrome staining. Serum levels of urea and creatinine were measured by commercial kits. ADR caused a significant increase in the serum level of urea compared with the control group. Interstitial fibrosis and TGF-β and collagen IV expression in the kidney tissue were also markedly increased in the ADR-treated rats. Administration of PM (600 and 1200 mg/kg) significantly improved the renal expression of these fibrotic markers and interstitial fibrosis, as well as serum levels of urea compared with the control group. Present results suggested that hydro-alcoholic extract of PM could improve kidney fibrosis induced by ADR administration.

阿霉素（ADR）是一种具有重要不良反应的抗癌药物，其中最重要的不良反应之一是肾病。大车前子( PM ) 是一种广泛使用的药用植物，具有许多药理特性。本研究的目的是研究PM提取物对 ADR 诱导的大鼠肾纤维化可能的肾脏保护作用。32 只雄性大鼠分为 4 组：对照组、ADR（5 毫克/千克，静脉注射）、PM 600 + ADR 和PM1200 + ADR。在研究的第 7 天注射 ADR。在实验的第 35 天结束时，收集左肾和血清样品。通过免疫组织化学检查胶原IV和转化生长因子(TGF)-β的表达。使用马森三色染色估计间质纤维化。尿素和肌酐的血清水平通过商业试剂盒测量。与对照组相比，ADR 导致血清尿素水平显着升高。在 ADR 治疗的大鼠中，肾组织中的间质纤维化和 TGF-β 和胶原蛋白 IV 的表达也显着增加。PM的管理与对照组相比，（600 和 1200 毫克/千克）显着改善了这些纤维化标志物的肾脏表达和间质纤维化，以及血清尿素水平。目前的结果表明PM 的水醇提取物可以改善 ADR 给药诱导的肾纤维化。