This present work is a part of the liquid discharges treatment topic by studying the removal performance for thermotolerant coliforms (FC) and fecal streptococci (F.Strep) by a local natural light green clay from Kenadsa (Bechar-Algeria) under continuous adsorption processes in a fixed-bed column. The study estimated the clay adsorbing efficiency by the adsorption technique for bacteria contaminating the dairy effluent by determining the bacterial load before and after treatment. The mean log counts per 100 ml for FC and F.Strep were assessed by MPN method on liquid medium. The clay material characterizations were made through X-ray diffraction, X fluorescence spectrometry and Fourier transform infrared spectroscopy analysis. Besides, some parameters were estimated such as the breakthrough time t_b (clay filter breakdown); the amount of the contaminating bacteria that was removed at the breakthrough time X_b and the exhaustion of disinfection capacity X_e; the total amount of contaminating bacteria flowing through the column X_total; and the total removal efficiency (Y). According to the XRD, XRF and FTIR results, the predominant mineral constituents were silicon dioxide, aluminum oxide, ferric oxide and magnesium oxide with rates of 59,44; 18,09; 7,79; and 3,87%, respectively, and hence, their classification among non-swelling clay minerals, illite is the major mineral group of this material. The results of the bacteriological analysis of raw dairy effluents showed an average bacterial load of 3,88 Log₁₀ and 4,1 Log₁₀ CFU/100 mL for FC and F.Strep, respectively, exceeding the thresholds set by the national and the international regulations. The results of the dairy effluents treated by the tested material have shown that the used clay has a relatively high adsorption property for the clay fixed-bed system (3 cm of bed height), expressed by a total removed efficiency Y (%) of FC and F.Strep used to evaluate the column performance ranging from 55 to 84%. It gives a higher log removal for FC and F.Strep (0.98–1.65 Log₁₀) reported from the first adsorption process, and a breakthrough time ranged from 100 to 250 min, which was inversely proportional to the initial bacterial load of discharges and also linked to the nature of the bacterial contaminants. When the breakthrough occurs earlier, the column service life will be shortened. For the studied parameters, the results of treated effluent complied with national and WHO regulations for unrestricted agricultural irrigation, otherwise, as authorized effluents to be discharged into nature without risks. These preliminary results are very promising at laboratory scale as an innovative green technology, treatment method respecting the environment and opens up prospects for the future, where the modification or the optimization of operating conditions such as the bed height of the fixed bed for adsorption, the volumetric flow rate or the clay structure like the particle size distribution of the adsorbents, known as one of the adsorbent classes endowed with an antimicrobial property, can improve the column performance, and further, the removal or even more the disinfection process by adsorption method.

本工作是液体排放处理主题的一部分，通过研究来自Kenadsa（Bechar-Algeria）的当地天然浅绿色粘土在连续吸附过程中对耐热大肠菌（FC）和粪便链球菌（F.Strep）的去除性能。固定床柱。该研究通过测定处理前后的细菌负荷来估计通过吸附技术对污染乳制品废水的细菌的吸附效率。FC 和 F.Strep 每 100 ml 的平均对数计数通过 MPN 方法在液体培养基上进行评估。通过X射线衍射、X荧光光谱和傅里叶变换红外光谱分析对粘土材料进行表征。此外，还估计了一些参数，例如突破时间 t _b（粘土过滤器故障）；在突破时间X _b和消毒能力耗尽X _e时去除的污染细菌数量；流过色谱柱的污染细菌_总量X_总量；和总去除效率（Y）。根据 XRD、XRF 和 FTIR 结果，主要矿物成分为二氧化硅、氧化铝、氧化铁和氧化镁，比例为 59,44；18,09; 7,79; 和 3.87%，因此，它们在非膨胀粘土矿物中的分类，伊利石是这种材料的主要矿物组。未加工乳制品流出物的细菌学分析结果显示平均细菌负荷为 3,88 Log ₁₀和 4,1 LogFC 和 F.Strep 分别为₁₀ CFU/100 mL，超过了国家和国际法规设定的阈值。经测试材料处理的乳制品废水结果表明，使用过的粘土对粘土固定床系统（床高3厘米）具有较高的吸附性能，用FC的总去除效率Y（%）表示和 F.Strep 用于评估色谱柱性能，范围为 55% 至 84%。它为 FC 和 F.Strep (0.98–1.65 Log ₁₀) 从第一次吸附过程报告，突破时间范围从 100 到 250 分钟，这与排放的初始细菌负荷成反比，也与细菌污染物的性质有关。越早发生突破，色谱柱使用寿命就会缩短。对于所研究的参数，处理后的出水结果符合国家和世界卫生组织关于无限制农业灌溉的规定，否则，作为授权的出水可以无风险地排放到大自然中。这些初步结果在实验室规模上非常有前景，作为一种创新的绿色技术、尊重环境的处理方法并开辟了未来的前景，其中操作条件的修改或优化，例如吸附固定床的床高，