The aim of this study was to investigate the host stage preference and functional response of Diaeretiella rapae (Hymenoptera: Braconidae) reared on Schizaphis graminum (Hemiptera: Aphididae). Firstly, host stage preference was determined under choice and no-choice tests. The parasitoid showed a preference for second instar nymphs of S. graminum in both choice and no-choice tests. Secondly, functional response was evaluated at five constant temperatures (10, 15, 20, 25, and 30 °C) and six host densities of second instar nymphs of S. graminum (2, 4, 8, 16, 32, and 64) during a 24 h period. The experiments were conducted at constant environmental conditions (25 ± 1ºC, 70 ± 5% RH and 16:8 h L:D photoperiod). Based on the logistic regression model, D. rapae exhibited a type II functional response at all five tested temperatures. The handling time was lowest at 30 °C (0.0263 h) and highest at 10 °C (2.5255 h). The searching efficiency of D. rapae varied at different temperatures. At 20 °C, the searching efficiency of the parasitoid was determined to be 0.0617 h⁻¹, which was significantly higher than other temperatures. The current results indicate that D. rapae can be used as a biological control agent of S. graminum at temperatures ranging between 20 and 25 °C, but additional data need to be collected in field and semi-field conditions.

本研究的目的是调查主机阶段偏好和的功能反应菜蚜茧蜂（膜翅目：茧蜂）饲养在麦二叉蚜（半翅目：蚜科）。首先，在选择和非选择测试下确定宿主阶段偏好。在选择和非选择测试中，寄生蜂表现出对禾本科二龄若虫的偏好。其次，在五个恒定温度（10、15、20、25 和 30 °C）和S. graminum的二龄若虫的六个寄主密度下评估功能响应（2、4、8、16、32 和 64）在 24 小时内。实验在恒定的环境条件（25 ± 1ºC、70 ± 5% RH 和 16:8 h L:D 光周期）下进行。基于逻辑回归模型，油菜花在所有五个测试温度下都表现出 II 型功能反应。处理时间在 30 °C (0.0263 h) 时最低，在 10 °C (2.5255 h) 时最高。不同温度下油菜的搜索效率不同。在 20 °C 时，寄生蜂的搜索效率确定为 0.0617 h ^-1，明显高于其他温度。目前的结果表明，油菜花可作为禾本科的生物防治剂 在 20 到 25 °C 的温度范围内，但需要在田间和半田间条件下收集额外的数据。