Double synonyms in the genetic code can be used as a tool to test competing hypotheses regarding ambigrammatic narnavirus genomes. Applying the analysis to recent observations of Culex narnavirus 1 and Zhejiang mosquito virus 3 ambigrammatic viruses indicates that the open reading frame on the complementary strand of the segment coding for RNA-dependent RNA polymerase does not code for a functional protein. Culex narnavirus 1 has been shown to possess a second segment, also ambigrammatic, termed ‘Robin’. We find a comparable segment for Zhejiang mosquito virus 3, a moderately diverged relative of Culex narnavirus 1. Our analysis of Robin polymorphisms suggests that its reverse open reading frame also does not code for a functional protein. We make a hypothesis about its role.

中文翻译：

---

遗传二元图的多态性

遗传密码中的双重同义词可用作测试有关双语法 narnavirus 基因组的竞争假设的工具。将分析应用于最近对库蚊 narnavirus 1 和浙江蚊子病毒 3 双重语法病毒的观察表明，编码依赖于 RNA 的 RNA 聚合酶的片段的互补链上的开放阅读框不编码功能性蛋白质。库蚊 narnavirus 1 已被证明具有第二个片段，也称为“罗宾”。我们发现了浙江蚊子病毒 3 的可比较片段，这是库蚊 narnavirus 1 的一个适度分化的亲戚。我们对 Robin 多态性的分析表明，它的反向开放阅读框也不编码功能性蛋白质。我们对其作用做出假设。