An eight-week study was conducted to determine requirements for chicory polysaccharides (CP) and their effects on the growth and immunity in the Chinese mitten crab Eriocheir sinensis H. Milne Edwards, 1853, followed by a challenge assay with the white spot syndrome virus (WSSV) for ten days. Crabs were separately fed with CP-free, 0.5% CP, 1% CP, and 4% CP diets. Results showed that crabs fed with 1% and 4% CP diets exhibited significant higher growth rate than that of controls (P < 0.05). The acid phosphatase (ACP), alkaline phosphatase (AKP), and peroxidase (POD) activities in the 1% CP group were clearly higher than those in the control groups (P < 0.05). The contents of glutathione (GSH) in 1% CP and 4% CP groups were significantly higher than those in the control group (P < 0.05). Malondialdehyde (MDA) contents in experimental groups, especially in the 1% CP group, decreased significantly relative to those in the control group (P < 0.05). When challenged by WSSV, the cumulative mortality was the lowest in the 4% CP group, whereas that in the control group was the highest (P < 0.05). Our study revealed that CP-supplemented diets enhanced specific growth, antioxidant activity, and anti-WSSV resistance in E. sinensis. Based on our findings, we suggest a CP diet content of 10 g kg–1 (1%).

中文翻译：

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菊苣多糖对中华绒螯蟹 Eriocheir sinensis H. Milne Edwards, 1853 (十足目: Brachyura: Varunidae) 生长、抗氧化活性和抗病性的影响

进行了一项为期八周的研究，以确定对菊苣多糖 (CP) 的需求及其对中华绒螯蟹 Eriocheir sinensis H. Milne Edwards 的生长和免疫的影响，1853 年，随后用白斑综合征病毒进行了攻击试验。 WSSV) 十天。螃蟹分别饲喂不含 CP、0.5% CP、1% CP 和 4% CP 的日粮。结果表明，投喂 1% 和 4% CP 日粮的螃蟹的生长速度明显高于对照组（P < 0.05）。1%CP组酸性磷酸酶（ACP）、碱性磷酸酶（AKP）和过氧化物酶（POD）活性明显高于对照组（P＜0.05）。1%CP组和4%CP组谷胱甘肽(GSH)含量显着高于对照组(P<0.05)。实验组尤其是1%CP组丙二醛（MDA）含量较对照组显着降低（P＜0.05）。当受到WSSV攻击时，4% CP组的累积死亡率最低，而对照组的累积死亡率最高（P < 0.05）。我们的研究表明，添加 CP 的日粮可增强 E. sinensis 的特定生长、抗氧化活性和抗 WSSV 抗性。根据我们的研究结果，我们建议 CP 日粮含量为 10 g kg–1 (1%)。我们的研究表明，添加 CP 的日粮可增强 E. sinensis 的特定生长、抗氧化活性和抗 WSSV 抗性。根据我们的研究结果，我们建议 CP 日粮含量为 10 g kg–1 (1%)。我们的研究表明，添加 CP 的日粮可增强 E. sinensis 的特定生长、抗氧化活性和抗 WSSV 抗性。根据我们的研究结果，我们建议 CP 日粮含量为 10 g kg–1 (1%)。