Among nanomaterials, we can now distinguish a special class called nanoflowers (NFs). These new nanostructures have aroused the interest of scientists due to the topographic features of nanolayers, the special location which allows a higher surface-to-volume ratio compared to classical spherical nanoparticles, thereby significantly increasing the efficiency of surface reactions for nanoflowers. The main value of nanoflowers is their action as enzyme stabilizers. A protein stability is usually enhanced by immobilization on a nanoflower surface through charge affinity and covalent bonds. The possibility of their use in vivo in biocatalysis, biosensors and medicine has been also investigated. We now report on the synthesis of two different nanoflowers: Cu nanoflowers and Fe³⁺ attached Cu nanoflowers and their interaction with two fluorescent probes, anilino-1-naphthalenesulfonic acid (ANS) and Fura 2, and two proteins, human serum albumin (HSA) and thrombin. Nanoflowers did not bind ANS, but bind efficiently to Fura 2 and both proteins. Modification of Cu–NFs by Fe³⁺ leads to significant changes in their binding capacity to fluorescent probe Fura 2 and both proteins. Their ability to bind fluorescent probe Fura 2 increased eightfold, and their ability to bind HSA and thrombin increased five times. Regarding Fe³⁺–Cu–NFs, a difference in binding between HSA and thrombin was found that can be explained by their structural features. Our data indicate the possibility of using studied nanoflowers for sorption of fluorescent probes and proteins.

在纳米材料中，我们现在可以区分一种特殊的类别，称为纳米花 (NFs)。由于纳米层的形貌特征，这些新的纳米结构引起了科学家的兴趣，与经典的球形纳米粒子相比，这种特殊的位置允许更高的表面体积比，从而显着提高了纳米花的表面反应效率。纳米花的主要价值在于它们作为酶稳定剂的作用。通常通过电荷亲和力和共价键固定在纳米花表面上来增强蛋白质稳定性。还研究了它们在体内生物催化、生物传感器和医学中使用的可能性。我们现在报告两种不同纳米花的合成：Cu 纳米花和 Fe ³⁺附着的铜纳米花及其与两种荧光探针苯胺基-1-萘磺酸 (ANS) 和 Fura 2 以及两种蛋白质、人血清白蛋白 (HSA) 和凝血酶的相互作用。Nanoflowers 不结合 ANS，但有效结合 Fura 2 和两种蛋白质。Fe ³⁺对Cu-NFs 的修饰导致其与荧光探针Fura 2 和两种蛋白质的结合能力发生显着变化。它们结合荧光探针 Fura 2 的能力增加了八倍，结合 HSA 和凝血酶的能力增加了五倍。关于 Fe ³⁺ -Cu-NFs，发现 HSA 和凝血酶之间的结合差异可以通过它们的结构特征来解释。我们的数据表明使用研究的纳米花来吸附荧光探针和蛋白质的可能性。