Abstract

Purpose

This study compared the effect of whole lung lavage (WLL) at different time-points early after exposure of the respiratory system to insoluble radioactive particles.

Materials and methods

Forty adult beagles were randomized into a control group and the 3-h, 8-h, 24-h, and 48-h lavage groups (n = 8). A canine model of acute lung injury was established by spraying a depleted uranium (DU) suspension using a superfine fiber bronchoscope, at a dose of 20 mg/kg. The lavage groups were subjected to WLL at 3 h, 8 h, 24 h, and 48 h post-DU exposure, while the control group received no treatment after exposure. Measurement of U in serum was performed using inductively coupled plasma mass spectrometry; measurements in the lavage fluid and left lung tissue were performed using inductively coupled plasma atomic emission spectrometry. The color of the lavage fluid was analyzed using colorimetry, and shadow changes in the lung were observed using chest computed tomography (CT).

Results

The lavage groups showed similarly increasing trends for serum U levels from DU exposure to 3 and 7 days after exposure; however, these values were significantly lower than those in the control group (p < .01). The U content in the lavage fluid was significantly higher in the 3-h group than in the 8-h, 24-h, and 48-h groups (p < .01), while that in the 8-h group was markedly higher than those in the 24-h and 48-h groups (p < .05). The average clearance rate of DU in the lungs varied in the range of 0.63‒7.06%. The U content in the left lung tissue of each lavage group was significantly lower than that in the control group (p < .01), while the content in the 8-h, 24-h, and 48-h groups was significantly higher than that in the 3-h group (p < .05). The colorimetric score of the lavage fluid in the 3-h group was significantly lower than those in the 8-h, 24-h, and 48-h groups (p < .05). Chest CT showed different degrees of consolidation and ground glass shadow changes in all groups. The score of the left lung shadow volume in the 3-h group was significantly lower than in the control, 8-h, 24-h, and 48-h groups (p < .01), while the score in the 8-h group was significantly higher than those in the 48-h and control groups (p < .05).

Conclusions

The best effect of WLL after exposure of the respiratory system to insoluble radioactive particles was achieved at 3 h, followed by 8 h; there was no difference in the effectiveness of lung lavage at 24 h and 48 h.

中文翻译：

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不同时间点早期全肺灌洗对促进肺内贫铀清除的影响

摘要

目的

本研究比较了呼吸系统暴露于不溶性放射性颗粒后早期不同时间点全肺灌洗 (WLL) 的效果。

材料和方法

四十只成年比格犬被随机分为对照组和 3 小时、8 小时、24 小时和 48 小时灌洗组（n  = 8）。通过使用超细纤维支气管镜以20 mg / kg的剂量喷雾贫铀（DU）悬浮液建立犬急性肺损伤模型。灌洗组在 DU 暴露后 3 小时、8 小时、24 小时和 48 小时接受 WLL，而对照组在暴露后不接受任何治疗。使用电感耦合等离子体质谱法测量血清中的 U；使用电感耦合等离子体原子发射光谱法对灌洗液和左肺组织进行测量。使用比色法分析灌洗液的颜色，并使用胸部计算机断层扫描 (CT) 观察肺部阴影变化。

结果

从 DU 暴露到暴露后 3 天和 7 天，灌洗组的血清 U 水平显示出类似的增加趋势；然而，这些值显着低于对照组（p  < .01）。3h组灌洗液U含量显着高于8h、24h和48h组（p  <0.01），而8h组显着高于比 24 小时和 48 小时组 ( p  < .05)。DU在肺部的平均清除率在0.63-7.06%之间变化。各灌洗组左肺组织U含量显着低于对照组（p < .01），而 8 小时、24 小时和 48 小时组的含量显着高于 3 小时组（p  < .05）。3-h 组灌洗液的比色评分显着低于 8-h、24-h 和 48-h 组（p  < .05）。胸部CT显示各组均有不同程度的实变和磨玻璃影改变。3-h 组左肺阴影体积评分显着低于对照组、8-h、24-h 和 48-h 组（p  < .01），而 8-h组显着高于 48 小时组和对照组（p  < .05）。

结论

呼吸系统暴露于不溶性放射性粒子后，WLL 的最佳效果是在 3 小时，其次是 8 小时；24 小时和 48 小时肺灌洗的有效性没有差异。