Abstract

The stirred ball mill (SBM) method is a common method applied in large-scale sludge disintegration. Many reports focus on the disintegration results of sludge or the influence of parameters on the anaerobic process of treated sludge, but there have been few studies on the mechanism of sludge disintegration by SBM. This study focused on the mechanism of sludge disintegration by the SBM method. The results show that the flocs and bacterial cell wall were well disrupted and that the cytoplasm flowed remarkably out of the cell. Although the size with the largest volume distribution was expected to decrease with increasing treatment time, the volume of particles with a size of 34.56–44.04 μm increased in the sample treated for 60 min. We observed a temperature increase in the reflocculation of the biomacromolecules. The maximal value of DD_COD was 18.6%, and the concentrations of proteins and nucleic acids reached peaks at 691.80 mg/L and 281.05 mg/L, respectively. The peptidoglycan concentration showed that only 25.4% of the cell wall was broken and dissolved into liquid. From the results, we show that SBM can effectively break flocs and bacterial cell walls, but the method has a low efficiency for breaking biomacromolecule fragments into the liquid phase.

Graphic Abstract

中文翻译：

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搅拌球磨机破碎过量污泥的机理研究——利用透射电镜观察和肽聚糖浓度测定

摘要

搅拌球磨机（SBM）法是大规模污泥破碎的常用方法。许多报道集中在污泥的分解结果或参数对处理后污泥厌氧过程的影响，但很少有关于SBM分解污泥机理的研究。本研究重点研究了 SBM 方法对污泥分解的机理。结果表明，絮体和细菌细胞壁被很好地破坏，细胞质显着流出细胞。尽管预计体积分布最大的尺寸会随着处理时间的增加而减小，但在处理 60 分钟的样品中，尺寸为 34.56-44.04 μm 的颗粒体积增加。我们观察到生物大分子再絮凝的温度升高。DD 的最大值_COD为 18.6%，蛋白质和核酸的浓度分别达到峰值 691.80 mg/L 和 281.05 mg/L。肽聚糖浓度显示只有 25.4% 的细胞壁被破坏并溶解成液体。结果表明，SBM 可以有效地破坏絮状物和细菌细胞壁，但该方法将生物大分子碎片破坏成液相的效率较低。

图形摘要