Electrochemical analysis of cobalt(III) protoporphyrin IX (CoP), synthesis and characterization of CoP nanoparticles, and signal amplification for biosensor development is presented. CoP was self-assembled into nanoparticles and then released to produce over 1000 electrochemically-detectable molecules for each protein target of interest, in this case monoclonal rabbit antibody. Anodic stripping voltammetry was utilized for quantitative and sensitive detection of CoP which correlated to target protein concentration. The CoP limit of detection was 4 nM and target protein was detected at 100 pM. This combination of nanoparticle and electrochemical signal amplification could allow for sensitive, inexpensive, and portable detection of protein biomarkers.

中文翻译：

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使用 Co(III) 原卟啉 IX 纳米颗粒和阳极溶出伏安法进行信号放大

介绍了钴 (III) 原卟啉 IX (CoP) 的电化学分析、CoP 纳米粒子的合成和表征以及用于生物传感器开发的信号放大。CoP 被自组装成纳米颗粒，然后释放以产生 1000 多个电化学可检测分子，用于每个感兴趣的蛋白质靶标，在这种情况下是单克隆兔抗体。阳极溶出伏安法用于定量和灵敏检测与目标蛋白浓度相关的 CoP。CoP 检测限为 4 nM，目标蛋白检测浓度为 100 pM。纳米粒子和电化学信号放大的这种组合可以实现对蛋白质生物标志物的灵敏、廉价和便携的检测。