Abstract

This work describes three phase dispersive liquid-liquid microextraction based on reverse micelles for the determination of Q-markers of cinnamic acids (caffeic acid, p-hydroxycinnamic acid, ferulic acid, and cinnamic acid) in traditional Chinese medicine followed by high performance liquid chromatography (HPLC) analysis. The variables influencing the procedure were optimized separately. Enrichment factors from 110 to 189, linearities from 1.2 to 960 ng mL⁻¹ with r² exceeding 0.9943, detection limits from 0.1 to 0.6 ng mL⁻¹, excellent precision with relative standard deviations below 5.6%, and good relative recoveries from 80.0% to 113.3% were achieved. In addition, the mechanism of the procedure was explored by comparison with conventional dispersive liquid-liquid microextraction at the optimum conditions. The results showed that the developed microextraction procedure improved the sensitivity and consumed less organic solvent and is a greener approach.

摘要

本工作描述了基于反胶束的三相分散液液微萃取，用于测定中药中肉桂酸（咖啡酸、对羟基肉桂酸、阿魏酸和肉桂酸）的 Q 标志物，然后采用高效液相色谱法(HPLC) 分析。影响程序的变量分别进行了优化。富集因子从 110 到 189，线性从 1.2 到 960 ng mL ^-1，r ²超过 0.9943，检测限从 0.1 到 0.6 ng mL ^-1、相对标准偏差低于 5.6% 的出色精密度，以及从 80.0% 到 113.3% 的良好相对回收率。此外，通过与常规分散液-液微萃取在最佳条件下进行比较，探索了该过程的机理。结果表明，开发的微萃取程序提高了灵敏度，消耗的有机溶剂更少，是一种更环保的方法。