Developing detailed rearing methods and describing the onset of gonadal differentiation in Red Shiners Cyprinella lutrensis could facilitate the development of novel techniques to control or enhance populations, enable toxicology studies, and help construct bioassays. In this study, we develop and report aquaculture practices for Red Shiner that ensure consistent year-round production in laboratory settings and evaluate the timing of sexual differentiation via histological gonad examinations. Our methods resulted in a mean of 56.00% (SD = 8.98%) survival through the larval stages of development, and we obtained spawns from captive-reared Red Shiners 138 d posthatch. Red Shiners are gonochoristic, and both ovaries and testes differentiate directly from undifferentiated gonads. Ovaries begin to differentiate in females 45 d posthatch, while testes begin differentiating in males 105 d posthatch. This study provides in-depth protocols for the closed-cycle aquaculture of Red Shiners and describes the gonadal differentiation and development of both sexes.

中文翻译：

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Red Shiner 水产养殖方案的发展和性腺分化

制定详细的饲养方法并描述红光鲤的性腺分化开始可以促进新技术的开发，以控制或增加种群，进行毒理学研究，并帮助构建生物测定。在这项研究中，我们开发并报告了 Red Shiner 的水产养殖实践，以确保实验室环境中全年一致的生产，并通过组织学性腺检查评估性别分化的时间。我们的方法导致幼虫整个发育阶段的平均存活率为 56.00% (SD = 8.98%)，并且我们获得了人工饲养的 Red Shiners 孵化后 138 天的产卵。Red Shiners 是生殖器官，卵巢和睾丸都直接与未分化的性腺区分开来。雌性孵化后 45 天卵巢开始分化，而雄性孵化后 105 天睾丸开始分化。