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Antihypernociceptive effects of Petersianthus macrocarpus stem bark on neuropathic pain induced by chronic constriction injury in rats
Inflammopharmacology ( IF 5.8 ) Pub Date : 2021-06-03 , DOI: 10.1007/s10787-021-00821-y
Francis Desire Tatsinkou Bomba 1, 2 , Telesphore Benoit Nguelefack 3 , Gangadhar Matharasala 2 , Ram Kumar Mishra 2 , Madhu Babu Battu 2 , Dharmarajan Sriram 2 , Albert Kamanyi 3 , Perumal Yogeeswari 2
Affiliation  

Petersianthus macrocarpus (Lecythidaceae) stem bark is traditionally used in West and Central Africa for the treatment of boils and pain. The present study examined the chemical composition of the aqueous and methanolic stem bark extracts of P. macrocarpus by liquid chromatography–electrospray ionization–mass spectrometry (LC–ESI–MS) . Their antinociceptive effect was evaluated using chronic constriction injury (CCI)-induced neuropathic pain in a rat model. On the ninth day post-surgery, the pain perception (allodynia and hyperalgesia) of the animals was assessed after the administration of aqueous and methanolic extracts at the doses of 100 and 200 mg/kg. In addition, the effect of the extracts was evaluated on nitric oxide activity and on the expression of pro-inflammatory cytokines (TNF-α, IL-1β, and NF-κB). The LC–ESI–MS analysis revealed the presence of ellagic acid as the major constituent in the methanol extract. Both extracts at the employed doses (100 and 200 mg/kg), significantly (p < 0.01 and p < 0.001) reduced the spontaneous pain, tactile and cold allodynia, and mechanical hyperalgesia. The methanolic extract used at the dose of 200 mg/kg significantly reduced the nitric oxide level (p < 0.001) and the gene expression levels of NF-κB (p < 0.05) and TNF-α (p < 0.01) in the brain. These data may indicate that stem bark extracts of P. macrocarpus possess a potent anti-hypernociceptive effect on CCI neuropathic pain. The inhibition of the nitric oxide pathway as well as the reduction in NF-κB and TNF-α gene expression in the brain may at least partially contribute to this effect. The results further support the use of this plant by traditional healers in pain conditions.



中文翻译:

大果皮对大鼠慢性压迫性神经病理性疼痛的镇痛作用

Petersianthus macrocarpus(Lecythidaceae)的茎皮传统上在西非和中非用于治疗疔疮和疼痛。本研究检查了P. macrocarpus的水性和甲醇茎皮提取物的化学成分通过液相色谱-电喷雾电离-质谱法 (LC-ESI-MS)。在大鼠模型中使用慢性压迫性损伤 (CCI) 诱发的神经性疼痛来评估它们的镇痛作用。在手术后第 9 天,在以 100 和 200 mg/kg 的剂量施用水提取物和甲醇提取物后评估动物的痛觉(异常性疼痛和痛觉过敏)。此外,还评估了提取物对一氧化氮活性和促炎细胞因子(TNF-α、IL-1β 和 NF-κB)表达的影响。LC-ESI-MS 分析表明鞣花酸是甲醇提取物中的主要成分。两种提取物在所用剂量(100 和 200 mg/kg)下显着(p  < 0.01 和p < 0.001) 减少了自发性疼痛、触觉和冷异常性疼痛以及机械性痛觉过敏。 以 200 mg/kg 的剂量使用的甲醇提取物显着降低了大脑中的一氧化氮水平 ( p < 0.001) 以及 NF-κB ( p  < 0.05) 和 TNF-α ( p < 0.01) 的基因表达水平。这些数据可能表明P. macrocarpus的茎皮提取物对 CCI 神经性疼痛具有有效的抗过度伤害感受作用。一氧化氮通路的抑制以及大脑中 NF-κB 和 TNF-α 基因表达的减少可能至少部分地促成了这种效应。结果进一步支持传统治疗师在疼痛情况下使用这种植物。

更新日期:2021-06-03
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