Abstract

Three endosulfan-degrading bacterial strains, Pseudomonas sp. KT1, Pseudomonas sp. KT2 and Staphylococcus sp. DKT, were isolated and investigated for their degradation under anaerobic conditions. These bacteria effectively degraded endosulfans and some related compounds. All of the isolates utilized nitrate as an electron acceptor and nitrogen source. Endosulfans degradation performances by a mixed culture of Pseudomonas sp. KT2 and Staphylococcus sp. DKT changed from 25.9 ± 4.5 to 34.0 ± 5.5% and were higher than the degradation by the each individual strain. Moreover, Pseudomonas sp. KT2 was the first pure culture capable of degrading a persistent compound, endosulfan sulfate, under anaerobic conditions. The determination of degradation metabolites showed that endosulfan diol, endosulfan ether and endosulfan lactone were formed during endosulfan degradation by Pseudomonas sp. KT1 and KT2. Dehalogenase extracted from mixed culture cells also revealed effective degradation and dechlorination. The results in this study show that a mixed culture was valuable for biodegradation of endosulfans and some of their relatives under anaerobic conditions.

中文翻译：

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假单胞菌混合培养对硫丹的厌氧降解。和葡萄球菌属。

摘要

三种硫丹降解细菌菌株，假单胞菌属。KT1，假单胞菌属。KT2 和葡萄球菌属。DKT 被分离出来并研究它们在厌氧条件下的降解情况。这些细菌有效地降解硫丹和一些相关化合物。所有的分离株都利用硝酸盐作为电子受体和氮源。假单胞菌混合培养物的硫丹降解性能。KT2 和葡萄球菌属。DKT 从 25.9 ± 4.5 变为 34.0 ± 5.5%，并且高于每个单独菌株的降解。此外，假单胞菌sp. KT2 是第一种能够在厌氧条件下降解持久性化合物硫丹硫酸盐的纯培养物。降解代谢物的测定表明，假单胞菌在降解硫丹的过程中形成了硫丹二醇、硫丹醚和硫丹内酯。KT1 和 KT2。从混合培养细胞中提取的脱卤酶也显示出有效的降解和脱氯作用。本研究的结果表明，混合培养物对于在厌氧条件下生物降解硫丹及其一些亲缘物种很有价值。