Changes in ghrelin, substance P and vasoactive intestinal peptide levels in the gastroduodenal mucosa of patients with morbid obesity,Neuropeptides

当前位置： X-MOL 学术 › Neuropeptides › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Changes in ghrelin, substance P and vasoactive intestinal peptide levels in the gastroduodenal mucosa of patients with morbid obesity
Neuropeptides ( IF 2.9 ) Pub Date : 2021-06-03 , DOI: 10.1016/j.npep.2021.102164
Unal Atas ₁ , Nuray Erin ₂ , Gokhan Tazegul ₁ , Gulsum Ozlem Elpek ₃ , Bulent Yildirim ₄

Affiliation

Aims

The aim of the study was to assess changes in levels of substance P (SP), vasoactive intestinal peptide (VIP) and ghrelin in the gastroduodenal mucosa of obese individuals, which has not been studied before.

Methods

Forty-six patients with a body mass index (BMI) of >40 kg/m² and 20 patients with a BMI of 18–25 kg/m² were included in the study. VIP and SP levels in the fundus, antrum and duodenal mucosa were measured in freshly frozen tissues using enzyme-linked immunosorbent assay (ELISA). Fasting levels of ghrelin in blood were also measured with ELISA. Tissue levels of ghrelin were assessed by immunohistochemical staining, and immunoreactivity scores were used for ghrelin evaluation in tissues.

Results

Antrum SP levels were higher in the obese group than in the control group. A significant number of obese patients had low VIP levels in the fundus and antrum. Intense ghrelin staining was observed in a limited number of cells in the mucosal area of the gastric fundus that was similar in the control and patient groups. In the antrum and duodenum, ghrelin staining was low in all the samples examined.

Conclusion

Here, we found that SP levels are increased, while VIP levels are decreased in the antrum of morbidly obese individuals. Previous studies show that SP increases gastroduodenal motility, that VIP slows it down, and that the gastric emptying rate is higher in obese individuals, preventing negative feedback mechanisms upon food intake. Therefore, increases in SP and decreases in VIP levels in the antrum may contribute to obesity by accelerating gastric emptying.

中文翻译：

病态肥胖患者胃十二指肠黏膜生长素释放肽、P物质和血管活性肠肽水平的变化

目标

该研究的目的是评估肥胖个体胃十二指肠黏膜中 P 物质 (SP)、血管活性肠肽 (VIP) 和生长素释放肽水平的变化，这在以前没有被研究过。

方法

该研究包括46 名体重指数 (BMI) > 40 kg/m ²的患者和 20 名 BMI 为 18-25 kg/m ²的患者。使用酶联免疫吸附试验 (ELISA) 在新鲜冷冻的组织中测量眼底、胃窦和十二指肠黏膜中的 VIP 和 SP 水平。还用 ELISA 测量了血液中的空腹饥饿素水平。通过免疫组织化学染色评估生长素释放肽的组织水平，并使用免疫反应性评分来评估组织中的生长素释放肽。