Selection of elite accessions is important to get the maximum quantity of bioactive compounds in medicinal plants. In this study, thirteen accessions of Plumbago zeylanica L., a medicinal herb, was studied for phytochemical and genetic diversity analysis. Phytochemical analysis showed that accession number IC-524441 contains the highest amount of flavonoids, phenolic, tannin and plumbagin content, it also possesses maximum antioxidant activity. Genetic diversity analysis showed that 15 SCoT (Start codon targeted) and 20 CBDP (CAAT Box Derived Polymorphism) primers produced 86 and 110 amplicons in total and average were 5.73 and 5.5 amplicons/primer respectively. PIC values ranged from 0.12 to 0.37 and average is 0.25/primer in the case of SCoT marker whereas, in the case of CBDP marker it was 0.15 to 0.59 with average of 0.26/primer. Cluster analysis showed that SCoT marker provides three clusters where cluster 1 and cluster 2 have only 2 accessions while cluster 3 contains nine accessions. Similarly, CBDP marker provides three clusters where cluster 1 contains only 1 accession and cluster 2 and cluster 3 contains six accessions in each. Further AMOVA analysis of SCoT marker possesses maximum variation of 8% among agro-ecological regions whereas in case of CBDP marker its 5%. From the present investigation, it was found that IC-524441 is an elite accession and contains highest amount of bioactive compound. Our results also demonstrate that both SCoT and CBDP markers are informative and can be utilized for evaluation of genetic relationships among Plumbago zeylanica L. accessions.

选择优良种质对于在药用植物中获得最大量的生物活性化合物很重要。在这项研究中，Plumbago zeylanica L. 的13 个种质 。是一种药草，用于植物化学和遗传多样性分析。植物化学分析表明，登记号IC-524441中黄酮类化合物、酚类、单宁和白花蕊的含量最高，抗氧化活性也最高。遗传多样性分析表明，15个SCoT（起始密码子靶向）和20个CBDP（CAAT盒衍生多态性）引物总共产生了86个和110个扩增子，平均分别为5.73和5.5个扩增子/引物。PIC 值范围为 0.12 至 0.37，SCoT 标记的平均值为 0.25/引物，而 CBDP 标记的平均值为 0.15 至 0.59，平均值为 0.26/引物。聚类分析显示 SCoT 标记提供三个聚类，其中聚类 1 和聚类 2 只有 2 个加入，而聚类 3 包含九个加入。相似地，CBDP 标记提供三个簇，其中簇 1 仅包含 1 个加入，簇 2 和簇 3 各包含 6 个加入。SCoT 标记的进一步 AMOVA 分析在农业生态区域之间具有 8% 的最大变异，而在 CBDP 标记的情况下为 5%。从目前的调查中发现，IC-524441 是一个精英种质，含有最高量的生物活性化合物。我们的结果还表明，SCoT 和 CBDP 标记都提供了信息，可用于评估之间的遗传关系。结果发现，IC-524441 是一种精英种质，含有最高量的生物活性化合物。我们的结果还表明，SCoT 和 CBDP 标记都提供了信息，可用于评估之间的遗传关系。结果发现，IC-524441 是一种精英种质，含有最高量的生物活性化合物。我们的结果还表明，SCoT 和 CBDP 标记都提供了信息，可用于评估之间的遗传关系。Plumbago zeylanica  L. 种质。