BACKGROUND:Blueberry is among the fastest growing fruit crops in the world, which is beneficial to human health and attracts extensive interests. In contrast to its rapid development and utilization, availability of molecular and genetic resources for blueberries are still scarce. OBJECTIVE:In present report, transcriptomic profiling of four widely cultivated varieties of Rabbiteye and Southern Highbush blueberries were characterized to assist the breeding programs. METHODS:Both de novo and reference-based assembly were conducted to generate the genetic resources that can be used in the further functional and breeding studies. RESULTS:De novo and reference-based assembly found average 136,350 and 158,123 non-redundant transcripts, respectively. Average 57,668 de novo assembled transcripts can be functionally annotated by homology search with different databases. We further detected 6,268 polymorphic simple sequence repeats, 566,913 high-quality single nucleotide polymorphisms and 88,662 insertion and deletions among the four varieties with comparison to a recently released reference genome of blueberry. Differentially expressed genes analysis showed that varieties of same species show less differences within species but larger differences between species. CONCLUSIONS:These comprehensive and high-quality genetic resources will contribute to a wide range of genetics and molecular breeding studies in blueberries.

中文翻译：

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Rabbiteye 和 Southern Highbush 蓝莓基于转录组的遗传资源

背景：蓝莓是世界上生长速度最快的水果作物之一，对人体健康有益，引起广泛关注。与快速开发利用相比，蓝莓分子遗传资源的可得性仍然不足。目的：在本报告中，对四种广泛栽培的 Rabbiteye 和 Southern Highbush 蓝莓品种的转录组分析进行了表征，以协助育种计划。方法：通过从头组装和参考组装来生成可用于进一步功能和育种研究的遗传资源。结果：从头和基于参考的组装分别发现平均 136,350 和 158,123 个非冗余转录本。平均 57, 668 个从头组装的转录本可以通过不同数据库的同源性搜索进行功能注释。与最近发布的蓝莓参考基因组相比，我们进一步检测到四个品种中的 6,268 个多态性简单序列重复、566,913 个高质量的单核苷酸多态性和 88,662 个插入和缺失。差异表达基因分析表明，同一物种的品种在物种内差异较小，但物种间差异较大。结论：这些全面、优质的遗传资源将有助于蓝莓的广泛遗传学和分子育种研究。与最近发布的蓝莓参考基因组相比，四个品种之间有 662 个插入和缺失。差异表达基因分析表明，同一物种的品种在物种内差异较小，但物种间差异较大。结论：这些全面、优质的遗传资源将有助于蓝莓的广泛遗传学和分子育种研究。与最近发布的蓝莓参考基因组相比，四个品种之间有 662 个插入和缺失。差异表达基因分析表明，同一物种的品种在物种内差异较小，但物种间差异较大。结论：这些全面、优质的遗传资源将有助于蓝莓的广泛遗传学和分子育种研究。