The ultraviolet resonance Raman (UVRR) spectra of the adenine-containing enzymatic redox cofactors nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) in aqueous solution of physiological concentration are compared with the aim of distinguishing between them and their building block adenine in potential co-occurrence in biological materials. At an excitation wavelength of 266 nm, the spectra are dominated by the strong resonant contribution from adenine, nevertheless bands assigned to vibrational modes of the nicotinamide and the flavin unit are found to appear at similar signal strength. Comparison of spectra measured at pH 7 with data obtained pH 10 and pH 3 shows characteristic changes when pH is increased or lowered, mainly due to deprotonation of the flavin and nicotinamide moieties, and protonation of the adenine, respectively.

比较生理浓度水溶液中含腺嘌呤的酶促氧化还原辅因子烟酰胺腺嘌呤二核苷酸（NADH）和黄素腺嘌呤二核苷酸（FAD）的紫外共振拉曼（UVRR）光谱，目的是区分它们及其构建块腺嘌呤。生物材料中的潜在共存。在 266 nm 的激发波长下，光谱主要由腺嘌呤的强共振贡献支配，但发现分配给烟酰胺和黄素单元振动模式的波段出现在相似的信号强度下。在 pH 7 下测量的光谱与在 pH 10 和 pH 3 下获得的数据的比较显示了当 pH 升高或降低时的特征变化，主要是由于黄素和烟酰胺部分的去质子化以及腺嘌呤的质子化，