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Construction of a high-density adzuki bean genetic map and evaluation of its utility based on a QTL analysis of seed size
Journal of Integrative Agriculture ( IF 4.8 ) Pub Date : 2021-06-01 , DOI: 10.1016/s2095-3119(20)63343-3
Li-xia WANG , Jie WANG , Gao-ling LUO , Xing-xing YUAN , Dan GONG , Liang-liang HU , Suhua WANG , Hong-lin CHEN , Xin CHEN , Xu-zhen CHENG

Adzuki bean (Vigna angularis (Willd.) Ohwi & Ohashi) is an annual cultivated leguminous crop commonly grown in Asia and consumed worldwide. However, there has been limited research regarding adzuki bean genetics, which has prevented the efficient application of genes during breeding. In the present study, we constructed a high-density genetic map based on whole genome re-sequencing technology and validated its utility by mining QTLs related to seed size. Moreover, we analyzed the sequences flanking insertions/deletions (InDels) to develop a set of PCR-based markers useful for characterizing adzuki bean genetics. A total of 2904 markers were mapped to 11 linkage groups (LGs). The total length of the map was 1365.0 cM, with an average distance between markers of 0.47 cM. Among the LGs, the number of markers ranged from 208 (LG7) to 397 (LG1) and the total distance ranged from 97.4 cM (LG9) to 155.6 cM (LG1). Twelve QTLs related to seed size were identified using the constructed map. The two major QTLs in LG2 and LG9 explained 22.1 and 18.8% of the total phenotypic variation, respectively. Ten minor QTLs in LG4, LG5 and LG6 explained 3.0–10.4% of the total phenotypic variation. A total of 9718 primer pairs were designed based on the sequences flanking InDels. Among the 200 selected primer pairs, 75 revealed polymorphisms in 24 adzuki bean germplasms. The genetic map constructed in this study will be useful for screening genes related to other traits. Furthermore, the QTL analysis of seed size and the novel markers described herein may be relevant for future molecular investigations of adzuki bean and will be useful for exploiting the mechanisms underlying legume seed development.



中文翻译:

基于种子大小QTL分析的高密度小豆遗传图谱构建及其效用评价

小豆 ( Vigna angularis)(Willd.) Ohwi & Ohashi) 是一种一年生种植的豆科作物,通常在亚洲种植并在全球范围内消费。然而,关于红豆遗传学的研究有限,这阻碍了基因在育种过程中的有效应用。在本研究中,我们构建了基于全基因组重测序技术的高密度遗传图谱,并通过挖掘与种子大小相关的 QTL 来验证其效用。此外,我们分析了插入/缺失 (InDels) 侧翼的序列,以开发一组基于 PCR 的标记,可用于表征小豆遗传学。总共 2904 个标记被映射到 11 个连锁群 (LGs)。地图的总长度为 1365.0 cM,标记之间的平均距离为 0.47 cM。在 LG 中,标记的数量从 208 (LG7) 到 397 (LG1) 不等,总距离从 97.4 cM (LG9) 到 155.6 cM (LG1)。使用构建的图谱鉴定了与种子大小相关的 12 个 QTL。LG2 和 LG9 中的两个主要 QTL 分别解释了总表型变异的 22.1% 和 18.8%。LG4、LG5 和 LG6 中的 10 个次要 QTL 解释了总表型变异的 3.0-10.4%。基于InDels侧翼的序列设计了总共9718个引物对。在选定的 200 个引物对中,75 个在 24 个红豆种质中显示了多态性。本研究构建的遗传图谱将有助于筛选与其他性状相关的基因。此外,

更新日期:2021-06-02
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