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Transcriptome analysis for understanding the mechanism of dark septate endophyte S16 in promoting the growth and nitrate uptake of sweet cherry
Journal of Integrative Agriculture ( IF 4.8 ) Pub Date : 2021-06-01 , DOI: 10.1016/s2095-3119(20)63355-x
Fan-lin WU , De-hui QU , Wei TIAN , Meng-yun WANG , Fei-yan CHEN , Ke-ke LI , Ya-dong SUN , Ying-hua SU , Li-na YANG , Hong-yan SU , Lei WANG

Sweet cherry is one of the most popular fresh fruits in the world. Previously, we isolated a soilborne dark septate endophyte (DSE) strain, S16, which promoted the growth of Gisela 5 sweet cherry rootstock. However, little is known about the molecular mechanism of the effect of S16 on the growth of sweet cherry. In this study, the physiological parameters and transcript profiles of sweet cherry roots were analyzed under S16 treatment compared with a control to elucidate the molecular mechanisms of the effect of this strain on sweet cherry growth. After inoculation with S16, sweet cherry seedlings exhibited more vigorous growth. Moreover, we identified 4249 differentially expressed genes (DEGs) between S16-treated plants and the control. Many of the DEGs are involved in pathways related to plant growth, such as cellular metabolic and plant hormone pathways. Additionally, some genes involved in nitrate regulation were also enriched; and these genes may be involved in the regulation of nitrate uptake in plants. Physiological index detection demonstrated that S16 could improve the nitrate assimilation of sweet cherry via NO3 transporters. This RNA-seq dataset provides comprehensive insight into the transcriptomic landscape to reveal the molecular mechanisms whereby the DSE influences the growth of sweet cherry.



中文翻译:

转录组分析了解深色有隔内生菌S16促进甜樱桃生长和硝酸盐吸收的机制

甜樱桃是世界上最受欢迎的新鲜水果之一。此前,我们分离了一种土传深色有隔内生菌 (DSE) 菌株 S16,它促进了 Gisela 5 甜樱桃砧木的生长。然而,关于S16对甜樱桃生长影响的分子机制知之甚少。在这项研究中,与对照相比,在 S16 处理下分析了甜樱桃根的生理参数和转录谱,以阐明该菌株对甜樱桃生长影响的分子机制。接种S16后,甜樱桃幼苗表现出更旺盛的生长。此外,我们在 S16 处理的植物和对照之间鉴定了 4249 个差异表达基因(DEG)。许多 DEG 参与与植物生长相关的途径,如细胞代谢和植物激素途径。此外,一些参与硝酸盐调控的基因也得到了富集;这些基因可能参与了植物对硝酸盐吸收的调控。生理指标检测表明S16能提高甜樱桃的硝酸盐同化作用通过NO 3 -转运蛋白。这个 RNA-seq 数据集提供了对转录组学景观的全面洞察,以揭示 DSE 影响甜樱桃生长的分子机制。

更新日期:2021-06-02
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