当前位置: X-MOL 学术Curr. Genomics › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Study on Transcriptional Responses and Identification of Ribosomal Protein Genes for Potential Resistance against Brown Planthopper and Gall Midge Pests in Rice
Current Genomics ( IF 2.6 ) Pub Date : 2021-01-31 , DOI: 10.2174/1389202922666210219113220
Mazahar Moin 1 , Anusree Saha 2 , Achala Bakshi 1 , Divya D 3 , Madhav M S 1 , Kirti P B 2, 3
Affiliation  

Background: Our previous studies have revealed the roles of ribosomal protein (RP) genes in the abiotic stress responses of rice.

Methods: In the current investigation, we examine the possible involvement of these genes in insect stress responses. We have characterized the RP genes that included both Ribosomal Protein Large (RPL) and Ribosomal Protein Small (RPS) subunit genes in response to infestation by two economically important insect pests, the brown planthopper (BPH) and the Asian rice gall midge (GM) in rice. Differential transcript patterns of seventy selected RP genes were studied in a susceptible and a resistant genotype of indica rice: BPT5204 and RPNF05, respectively. An in silico analyses of the upstream regions of these genes also revealed the presence of cis-elements that are associated with wound signaling.

Results: We identified the genes that were up or downregulated in either one of the genotypes, or both of them after pest infestation. The transcript patterns of a majority of the genes were found to be temporally-regulated by both the pests. In the resistant RPNF05, BPH infestation activated RPL15, L51 and RPS5a genes while GM infestation induced RPL15, L18a, L22, L36.2, L38, RPS5, S9.2 and S25a at a certain point of time. These genes that were particularly upregulated in the resistant genotype, RPNF05, but not in BPT5204 suggest their potential involvement in plant resistance against either of the two pests studied.

Conclusion: Taken together, RPL15, L51, L18a, RPS5, S5a, S9.2, and S25a appear to be the genes with possible roles in insect resistance in rice.



中文翻译:

水稻褐飞虱和瘿蚊潜在抗性核糖体蛋白基因的转录反应和鉴定研究

背景:我们之前的研究揭示了核糖体蛋白(RP)基因在水稻非生物胁迫反应中的作用。

方法:在当前的研究中,我们研究了这些基因可能参与昆虫应激反应。我们对包括核糖体蛋白大 (RPL) 和核糖体蛋白小 (RPS) 亚基基因在内的 RP 基因进行了表征,以响应两种经济上重要的害虫——褐飞虱 (BPH) 和亚洲稻瘿蚊 (GM) 的侵染。在米饭中。研究了 70 个选定的 RP 基因在籼稻的易感基因型和抗性基因型:BPT5204 和 RPNF05 中的差异转录模式。对这些基因上游区域的计算机分析还揭示了与伤口信号传导相关的顺式元件的存在。

结果:我们鉴定出在害虫侵扰后任一基因型或两者中上调或下调的基因。发现大多数基因的转录模式受到两种害虫的时间调节。在抗性 RPNF05 中,BPH 感染激活 RPL15、L51 和 RPS5a 基因,而 GM 感染则在某个时间点诱导 RPL15、L18a、L22、L36.2、L38、RPS5、S9.2 和 S25a 基因。这些基因在抗性基因型 RPNF05 中尤其上调,但在 BPT5204 中却没有,表明它们可能参与植物对所研究的两种害虫的抗性。

结论:总的来说,RPL15、L51、L18a、RPS5、S5a、S9.2 和 S25a 似乎是在水稻抗虫性中可能发挥作用的基因。

更新日期:2021-01-31
down
wechat
bug