UHPLC combined with Fourier-transform ion cyclotron resonance MS metabonomic approach was employed to screen the differential components between normal rats and yeast-induced pyrexia rats after an oral administration of Gegenqinlian decoction (GQLD). Nine compounds, namely puerarin, daidzein, baicalin, wogonoside, wogonin, berberine, palmatine, jateorhizine, and coptisine, were identified as differential components in the plasma. A rapid, sensitive, selective, and accurate UHPLC–MS method was developed and fully validated for the simultaneous determination of the screened components in rat plasma after an oral administration of GQLD. The values for the limit of quantification ranged from 0.025 to 5.0 ng/mL. The inter- and intra-day precision of all analytes was ≤10.7%, with an accuracy of ≤10.5%. Good extraction recovery and matrix effects were also obtained. The method was successfully applied to a comparative pharmacokinetic study of GQLD in normal and pyrexia rats. The results showed that the pharmacokinetic behavior of the analytes was changed in pyrexia rats compared to normal rats. These results could provide beneficial guidance for clinical applications of GQLD.

中文翻译：

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UHPLC-FT-ICR-MS和UHPLC-MS/MS筛选葛根芩连汤差异成分及其在正常和发热大鼠体内的药代动力学比较

采用UHPLC结合傅里叶变换离子回旋共振MS代谢组学方法筛选口服葛根芩连汤（GQLD）后正常大鼠和酵母致发热大鼠的差异成分。九种化合物，即葛根素、黄豆苷元、黄芩苷、汉黄芩苷、汉黄芩素、小檗碱、巴马汀、黄连素和黄连素，被鉴定为血浆中的差异成分。开发并充分验证了一种快速、灵敏、选择性和准确的 UHPLC-MS 方法，用于同时测定口服 GQLD 后大鼠血浆中的筛选成分。定量限的值范围为 0.025 至 5.0 ng/mL。所有分析物的日间和日内精密度≤10.7%，准确度≤10.5%。还获得了良好的提取回收率和基质效应。该方法已成功应用于正常和发热大鼠 GQLD 的比较药代动力学研究。结果表明，与正常大鼠相比，发热大鼠中分析物的药代动力学行为发生了变化。这些结果可为GQLD的临床应用提供有益的指导。