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Screening and cocktail optimization using experimental mixture design: enzymatic saccharification as a biological pretreatment strategy
Biofuels, Bioproducts and Biorefining ( IF 3.9 ) Pub Date : 2021-06-02 , DOI: 10.1002/bbb.2251
Vanessa Elisa Pinheiro 1, 2 , Ilona Sárvári Horváth 2 , Magnus Lundin 2 , Maria de Lourdes Teixeira de Moraes Polizeli 1, 3
Affiliation  

Biofuels contribute environment-friendly, renewable energy, minimizing dependence on fossil energy. The pretreatment of biomass is a practical step that accelerates and facilitates the hydrolysis of complex materials. This work aimed to screen, select, and study cocktail formulations for biomass hydrolysis, where the enzymes were provided both from a commercial source (Trichoderma reesei, Novozymes®) and through the cultivation of Aspergillus brasiliensis and Aspergillus tamarii Kita. Experimental mixture designs were used to optimize the enzymatic conversion of substrates into simple sugars. A crude extract rich in amylase (AAB) had a significant favorable influence on cornmeal hydrolysis by maximizing the yield of reducing sugars (RS) (173 μmol mL–1). Celluclast™, rich in cellulase, significantly affected the hydrolysis of banana peel, maximizing the RS yield (175 μmol mL–1). Variable degrees of enzyme synergism were evident from statistical analysis of the biomass hydrolysis. © 2021 Society of Chemical Industry and John Wiley & Sons, Ltd

中文翻译:

使用实验混合物设计进行筛选和鸡尾酒优化:酶促糖化作为生物预处理策略

生物燃料有助于环保、可再生能源,最大限度地减少对化石能源的依赖。生物质的预处理是加速和促进复杂材料水解的实用步骤。这项工作的目的是屏幕上,选择,和研究鸡尾酒制剂用于生物质的水解,其中所述酶购自商业来源(提供二者里氏木霉,Novozymes®)和通过的培养曲霉巴西曲霉实验混合物设计用于优化底物向单糖的酶促转化。富含淀粉酶 (AAB) 的粗提物通过最大化还原糖 (RS) 的产量 (173 μmol mL –1 )对玉米面水解产生显着的有利影响。Celluclast™ 富含纤维素酶,显着影响香蕉皮的水解,使 RS 产量最大化 (175 μmol mL –1 )。从生物质水解的统计分析中可以明显看出不同程度的酶协同作用。© 2021 化学工业协会和 John Wiley & Sons, Ltd
更新日期:2021-06-02
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