Abstract

Several side effects and drug resistance accompany the current therapies for Leishmaniasis. Nanoliposomal curcumin is applied as a new therapy approach instead of current therapy. In this study, nanoliposomal curcumin was prepared using thin-film hydration method and characterized based on encapsulation efficiency, size, and zeta potential. Curcumin was successfully loaded into nanoliposomes with an encapsulation efficiency of 92%. The surface charge of the nanoparticle was neutral, and the size of nanoparticle was 176.5 nm. Nanoliposomal curcumin is in spherical shape without any agglomeration. Cell viability assay was performed on HFF cell line to show biocompatibility of liposome nanoparticles. Anti-Leishmanial effect of different concentrations of liposomal curcumin (0.05–30 μg mL⁻¹) and amphotericin B (25 μg mL⁻¹) were studied on Leishmania major [MRHO/IR/75/ER] at various hours (24, 48, and 72) using hemocytometer technique. Nanoliposomal curcumin inhibitory concentration (IC50) at hours 24, 48, and 72 were 6.41, 3.8, and 2.33 µg mL⁻¹, respectively. As prepared nanoliposomal curcumin showed a significant antileishmanial effect and induced a better and more tangible effect on the survival of L. major promastigotes and could be suitable candidates for further investigations.

摘要

目前的利什曼病疗法伴随着一些副作用和耐药性。纳米脂质体姜黄素被用作一种新的治疗方法，而不是目前的治疗方法。在这项研究中，纳米脂质体姜黄素是使用薄膜水合法制备的，并基于封装效率、尺寸和 zeta 电位进行表征。姜黄素以 92% 的包封率成功加载到纳米脂质体中。纳米粒子表面电荷呈中性，纳米粒子大小为176.5 nm。纳米脂质体姜黄素呈球形，没有任何团聚。对 HFF 细胞系进行细胞活力测定以显示脂质体纳米颗粒的生物相容性。不同浓度脂质体姜黄素（0.05–30 μg mL ^-1) 和两性霉素 B (25 μg mL ^-1 )在不同时间（24、48 和 72 小时）使用血细胞计数器技术对主要利什曼原虫[MRHO/IR/75/ER] 进行研究。纳米脂质体姜黄素抑制浓度 (IC50) 在 24、48 和 72 小时分别为 6.41、3.8 和 2.33 µg mL ^-1。制备的纳米脂质体姜黄素显示出显着的抗利什曼原虫作用，并对主要前鞭毛体的存活产生更好和更明显的影响，可能是进一步研究的合适候选者。