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Joint reconstruction of an in-focus image and of the background signal in in-line holographic microscopy
Optics and Lasers in Engineering ( IF 4.6 ) Pub Date : 2021-06-01 , DOI: 10.1016/j.optlaseng.2021.106691
Anthony Berdeu , Thomas Olivier , Fabien Momey , Loïc Denis , Frédéric Pinston , Nicolas Faure , Corinne Fournier

In-line digital holography is a simple yet powerful tool to image absorbing and/or phase objects. However, the holograms of interest are corrupted by the background signal due to unwanted scattering elements located in the optical path.

Using only two holograms of the same object, shifted to different locations, an inverse problems approach is applied to jointly estimate the complex transmittance of the sample and the contribution of the interferent background signal at the sensor plane. Experimental results with stained bacteria are presented and show improved reconstructions of the sample while also accounting for the background contribution.



中文翻译:

在线全息显微镜中聚焦图像和背景信号的联合重建

在线数字全息是一种简单而强大的工具,可以对吸收和/或相位物体进行成像。然而,由于位于光路中的不需要的散射元件,感兴趣的全息图被背景信号破坏。

仅使用移动到不同位置的同一物体的两个全息图,应用逆问题方法来联合估计样品的复透射率和传感器平面上干扰背景信号的贡献。展示了染色细菌的实验结果,显示了改进的样本重建,同时也考虑了背景贡献。

更新日期:2021-06-01
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