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Comparison of Macrophomina phaseolina inoculation techniques for screening sunflower and soybean germplasm in a controlled environment
Canadian Journal of Plant Pathology ( IF 2 ) Pub Date : 2021-07-15 , DOI: 10.1080/07060661.2021.1937321
T. Talapov 1 , S. Yuceer 2 , O. Dedecan 1 , O. Demirel 1 , C. Can 1
Affiliation  

Abstract

Macrophomina phaseolina is a polyphagous phytopathogenic fungus that causes serious yield losses in economically important crop plants. The most appropriate control measure for this fungus is the utilization of resistance sources. To achieve this a fast and reliable screening method, in addition to a characterized pathogen population, is a prerequisite to accelerate breeding programmes. The aim of this study was to develop an efficient inoculation method for soybean and sunflower seedlings and to test the effect of inoculum maturity on disease development under controlled environmental conditions. Three inoculation methods, stem-tape, cut-stem and toothpick, and two inoculum types, hyphal and microsclerotial, were tested on sunflower and soybean seedlings. The fastest disease development on sunflower was obtained with the cut-stem and stem-tape inoculation methods using hyphal inoculum, and both methods had high repeatability. The cut-stem method, however, is not applicable for germplasm screening studies due to the predisposition of sunflower seedlings to pathogen attack. Application of the stem-tape inoculation method was useful for a variety of host reaction studies and for rapid germplasm screening. In soybean, the cut-stem inoculation method yielded consistent results in pathogenicity and variety reaction studies, but the stem-tape and toothpick inoculation methods had poor repeatability and long incubation periods, respectively. The cut-stem method in soybean and the stem-tape method in sunflower appear to be the most appropriate for pathogenicity and rapid germplasm screening studies with M. phaseolina under controlled conditions.



中文翻译:

在受控环境下筛选向日葵和大豆种质的小球藻接种技术比较

摘要

小豆豉是一种多食性植物病原真菌,在具有重要经济意义的作物中造成严重的产量损失。这种真菌最合适的控制措施是利用抗性源。为了实现这一目标,除了具有特征的病原体种群外,快速可靠的筛选方法是加快育种计划的先决条件。本研究的目的是开发一种有效的大豆和向日葵幼苗接种方法,并在受控环境条件下测试接种成熟度对疾病发展的影响。在向日葵和大豆幼苗上测试了三种接种方法,茎带、切茎和牙签,以及两种接种类型,菌丝和微菌核。使用菌丝接种的切茎和茎带接种方法在向日葵上病害发展最快,并且这两种方法具有较高的重复性。然而,由于向日葵幼苗易受病原体侵袭,切茎法不适用于种质筛选研究。茎带接种法的应用可用于各种宿主反应研究和快速种质筛选。在大豆中,切茎接种法在致病性和品种反应研究中产生了一致的结果,但茎带接种法和牙签接种法分别具有重复性差和潜伏期长的问题。大豆的切茎法和向日葵的茎带法似乎最适合致病性和快速种质筛选研究。M. 菜豆在受控条件下。

更新日期:2021-07-15
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