For the estimation of genetic diversity and population structure, 23 accessions of Leucaena leucocephala were characterized using morphological and molecular markers. A medium to high degree of genetic variability was calculated among these accessions based on eight quantitative traits. Furthermore, a cluster analysis based on morphological characteristics grouped the 23 Leucaena accessions into five groups based on their similarities. Furthermore, the principal component analysis revealed that eight quantitative traits contributed for 82.57% of the total variability. Eleven simple sequence repeats (SSR) markers showed a high degree of polymorphism among the 23 L. leucocephala accessions. The polymorphic information contents (PIC) for these 11 markers were estimated and revealed that SSR723 had higher PIC value (0.90). Also, the dendrogram generated based on 11 markers distinctively demarcated most of the L. leucocephala accessions into different clusters indicating its vast genetic diversity in the population. A population structure study of these 23 L. leucocephala accessions showed the existence of two gene pools. Also, analysis of molecular variance showed that there was 13% variability between populations, while there was 87% variability within the population. The first and second coordinates of principal coordinate analysis unconditionally distributed these accessions into four coordinates based on their similarity. Overall, the high degree of genetic variability described by morphological and molecular markers in L. leucocephala can be used as a basic tool for assessing genetic diversity and linkage mapping in Leucaena genetic improvement programs.

为了估计遗传多样性和种群结构，使用形态学和分子标记对 23 份银合欢种质进行了表征。根据八个数量性状计算了这些种质之间的中等至高度遗传变异性。此外，基于形态特征的聚类分析将 23 个银合欢种质根据它们的相似性分为五组。此外，主成分分析显示，8 个数量性状占总变异的 82.57%。11 个简单序列重复 (SSR) 标记在 23 种白头虱中表现出高度的多态性加入。对这 11 个标记的多态信息含量 (PIC) 进行了估计，结果表明 SSR723 具有更高的 PIC 值 (0.90)。此外，基于 11 个标记生成的树状图将大部分白头蜂种质明确划分为不同的簇，表明其在种群中具有巨大的遗传多样性。这些 23 L. leucocephala 的种群结构研究种质显示存在两个基因库。此外，分子方差分析表明，种群之间存在 13% 的变异性，而种群内存在 87% 的变异性。主坐标分析的第一和第二坐标根据它们的相似性将这些种质无条件地分配到四个坐标中。总体而言，由形态学和分子标记描述的 L. leucocephala 中的高度遗传变异可用作评估 Leucaena 遗传改良计划中遗传多样性和连锁作图的基本工具。