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Effects of 5-aminolevulinic acid on Anthocyanin synthesis in Vitis Vinifera ‘Crimson Seedless’ grapes at the transcriptomics level
The Journal of Horticultural Science and Biotechnology ( IF 1.9 ) Pub Date : 2021-05-30 , DOI: 10.1080/14620316.2021.1930589
Zhijun Zhang 1 , Lianling Liu 1 , Xinyi Chang 1 , Wang He 1 , Jingjing Liu 1 , Baolong Zhao 2 , Junli Sun 1
Affiliation  

ABSTRACT

In order to study the effect of exogenous 5-aminolevulinic acid (ALA) on the expression of genes associated with anthocyanin biosynthesis in the transcriptome of ‘Crimson Seedless’ grape (Vitis vinifera) peel, sequencing technology was used to compare the transcriptomes from water and ALA treated ‘Crimson Seedless’ grapes. Transcriptome sequencing was performed at two periods (d5 and d15) after peel treatment. The enrichment analysis of the Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathways indicated that the metabolic pathways induced by exposure to ALA were mainly the phenylpropanoid biosynthesis, flavonoid biosynthesis, anthocyanin biosynthesis, phenylalanine metabolism, and plant hormone signal transduction pathways. In the anthocyanin synthesis pathway, ALA treatment appeared to mainly regulate the phenylalanine ammonia-lyase, cinnamate 4-hydroxylase and chalcone synthase genes in the upstream pathway of anthocyanin synthesis, and the dihydroflavonol-4-reductase gene in the downstream pathway, with the effect being most obvious at d5.This study reveals the mechanism by which exogenous ALA promotes anthocyanin synthesis at the molecular level, and lays the foundation for further study of the physiological role of ALA.



中文翻译:

5-氨基乙酰丙酸在转录组学水平对“深红无核”葡萄花青素合成的影响

摘要

为了研究外源性5-氨基乙酰丙酸(ALA)对'Crimson Seedless'葡萄(Vitis vinifera)转录组中花青素生物合成相关基因表达的影响) 果皮,测序技术用于比较水和 ALA 处理的“深红色无籽”葡萄的转录组。在剥离处理后的两个时期(d5 和 d15)进行转录组测序。京都基因与基因组百科全书(KEGG)途径的富集分析表明,ALA暴露诱导的代谢途径主要是苯丙烷生物合成、黄酮生物合成、花青素生物合成、苯丙氨酸代谢和植物激素信号转导途径。在花色苷合成途径中,ALA处理似乎主要调节花色苷合成上游途径中的苯丙氨酸解氨酶、肉桂酸4-羟化酶和查尔酮合酶基因,以及下游途径中的二氢黄酮醇-4-还原酶基因,具有作用在 d5 时最为明显。

更新日期:2021-05-30
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