The morphological and functional state of the reproductive system was studied in male outbred rats (SD stock) and male F1(CBA×C57BL/6) mice after long-term (3 months) methotrexate administration. The drug was administered subcutaneously once a week for 4 weeks, the dose for male rats was 1 mg/kg, for male mice 2.2 mg/kg. It was found that male rats retained the ability to conceive, their reproductive potential was not limited by increased risk of embryo death. At the same time, signs of astheno- and pathospermia were revealed. The testicular tissue was characterized by reduced content of the sources of the proliferative pool of spermatogenesis. In mice treated with methotrexate, increased content of DNA breaks was detected in the testicular cells.

在长期（3个月）服用甲氨蝶呤后，研究雄性远交大鼠（SD股票）和雄性F1（CBA×C57BL/6）小鼠生殖系统的形态和功能状态。该药每周一次皮下给药，持续4周，雄性大鼠的剂量为1 mg/kg，雄性小鼠的剂量为2.2 mg/kg。结果发现，雄性大鼠保留了受孕的能力，它们的生殖潜力不受胚胎死亡风险增加的限制。同时，显示出弱精症和病理精子症的迹象。睾丸组织的特征是精子发生增殖池来源的含量减少。在用甲氨蝶呤治疗的小鼠中，在睾丸细胞中检测到 DNA 断裂的含量增加。