Objective. To investigate the potential mechanism of action of Yi-Qi-Huo-Xue-Tong-Luo formula (YQHXTLF) in the treatment of diabetic peripheral neuropathy (DPN). Methods. Network pharmacology and molecular docking techniques were used in this study. Firstly, the active ingredients and the corresponding targets of YQHXTLF were retrieved using the Traditional Chinese Medicine Systems Pharmacology (TCMSP) platform; subsequently, the targets related to DPN were retrieved using GeneCards, Online Mendelian Inheritance in Man (OMIM), Pharmgkb, Therapeutic Target Database (TTD) and Drugbank databases; the common targets of YQHXTLF and DPN were obtained by Venn diagram; afterwards, the “YQHXTLF Pharmacodynamic Component-DPN Target” regulatory network was visualized using Cytoscape 3.6.1 software, and Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed on the potential targets using R 3.6.3 software. Finally, molecular docking of the main chemical components in the PPI network with the core targets was verified by Autodock Vina software. Results. A total of 86 active ingredients and 229 targets in YQHXTLF were screened, and 81 active ingredients and 110 targets were identified to be closely related to diabetic peripheral neuropathy disease. PPI network mapping identified TP53, MAPK1, JUN, and STAT3 as possible core targets. KEGG pathway analysis showed that these targets are mostly involved in AGE-RAGE signaling pathway in diabetic complications, TNF signaling pathway, and MAPK signaling pathway. The molecular docking results showed that the main chemical components of YQHXTLF have a stable binding activity to the core pivotal targets. Conclusion. YQHXTLF may act on TP53, MAPK1, JUN, and STAT3 to regulate inflammatory response, apoptosis, or proliferation as a molecular mechanism for the treatment of diabetic peripheral neuropathy, reflecting its multitarget and multipathway action, and providing new ideas to further uncover its pharmacological basis and mechanism of action.

中文翻译：

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益气活血通络方治疗糖尿病周围神经病变的网络药理学与分子对接研究

客观。探讨益气活血通络方(YQHXTLF)治疗糖尿病周围神经病变(DPN)的潜在作用机制。方法. 本研究采用网络药理学和分子对接技术。首先，利用中药系统药理学（TCMSP）平台检索到益气活血方的有效成分及相应靶点；随后，使用 GeneCards、Online Mendelian Inheritance in Man (OMIM)、Pharmgkb、Therapeutic Target Database (TTD) 和 Drugbank 数据库检索与 DPN 相关的目标；YQHXTLF和DPN的共同目标通过维恩图得到；随后，使用Cytoscape 3.6.1软件可视化“YQHXTLF Pharmacodynamic Component-DPN Target”调控网络，并使用R对潜在靶点进行基因本体（GO）富集分析和京都基因与基因组百科全书（KEGG）通路分析。 3.6.3 软件。最后，结果。共筛选出益气活血方的86个有效成分和229个靶点，确定81个有效成分和110个靶点与糖尿病周围神经病变密切相关。PPI 网络映射将 TP53、MAPK1、JUN 和 STAT3 确定为可能的核心目标。KEGG通路分析表明，这些靶点主要参与糖尿病并发症中的AGE-RAGE信号通路、TNF信号通路和MAPK信号通路。分子对接结果表明，YQHXTLF的主要化学成分对核心关键靶点具有稳定的结合活性。结论. YQHXTLF 可能作用于 TP53、MAPK1、JUN 和 STAT3 调节炎症反应、细胞凋亡或增殖作为治疗糖尿病周围神经病变的分子机制，体现其多靶点和多通路作用，为进一步揭示其药理基础提供新思路和作用机制。