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High percentage of dietary soybean meal inhibited growth, impaired intestine healthy and induced inflammation by TLR-MAPK/NF-κB signaling pathway in large yellow croaker (Larimichthys crocea)
Aquaculture Reports ( IF 3.7 ) Pub Date : 2021-05-27 , DOI: 10.1016/j.aqrep.2021.100735
Wan-Qing Zhu , Xiao-Qin Yuan , Hong-Jie Luo , Jian-Chun Shao , Xin-Hua Chen

Soybean meal (SBM) is a high-quality protein source and widely used in aquafeeds. However, high percentage of dietary SBM would make negative influences on fish. Here, we evaluated the effects of dietary high level of SBM on growth, intestine healthy and intestinal inflammation in large yellow croaker. Two isonitrogenous (450 g kg−1) and isolipidic (100 g kg−1) diets were prepared, in which SBM was added at 0 or 45 %. An 8-week feeding trail was conducted by feeding fish (initial weight 18.2 ± 0.8 g) with these two diets. After feeding trail, SBM0 group had significantly enhanced growth performance, antioxidant capacity and digestive enzyme activities compared with SBM45 group (P < 0.05). Dietary SBM45 caused intestine injury symptoms and decreased intestine villus length of fish. In addition, dietary SBM45 induced intestinal inflammation of large yellow croaker, manifested as significant upregulation of pro-inflammatory cytokines and downregulation of anti- inflammatory cytokines (P < 0.05). The increased inflammatory response of SBM45 group may be due to activation of TLR-MAPK/NF-κB signaling pathway, as the mRNA expression levels of TLRs and protein levels of c-Rel, P65 and ERK were significantly increased in SBM45 group (P < 0.05). All these results indicated that high percentage of dietary soybean meal could inhibit growth, impair intestine healthy and induce intestinal inflammation possibly by TLR-MAPK/NF-κB signaling pathway in large yellow croaker.



中文翻译:

高比例的膳食豆粕通过 TLR-MAPK/NF-κB 信号通路抑制大黄鱼 ( Larimichthys crocea ) 的生长、损害肠道健康并诱导炎症

豆粕 (SBM) 是一种优质蛋白质来源,广泛用于水产饲料。然而,高比例的膳食 SBM 会对鱼类产生负面影响。在这里,我们评估了高水平 SBM 对大黄鱼生长、肠道健康和肠道炎症的影响。制备了两种异氮(450 g kg -1)和异脂(100 g kg -1)日粮,其中添加了 0 或 45% 的 SBM。通过用这两种饮食喂养鱼(初始体重 18.2 ± 0.8 g)进行了为期 8 周的喂养试验。饲养试验后,SBM0组与SBM45组相比,生长性能、抗氧化能力和消化酶活性显着增强(P< 0.05)。日粮 SBM45 引起肠道损伤症状并减少鱼的肠绒毛长度。此外,日粮SBM45诱导大黄鱼肠道炎症,表现为促炎细胞因子显着上调和抗炎细胞因子下调(P < 0.05)。SBM45组炎症反应增强可能与TLR-MAPK/NF-κB信号通路激活有关,SBM45组TLRs mRNA表达水平和c-Rel、P65、ERK蛋白水平显着升高(P < 0.05)。所有这些结果表明,高百分比的膳食豆粕可能通过大黄鱼中的TLR-MAPK /NF-κB信号传导途径抑制生长,损害肠道健康并诱导肠道炎症。

更新日期:2021-05-28
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