Agaricus bisporus cultivation is based on a selective substrate prepared by a meticulous composting process where thermophilic and/or thermotolerant fungi might play an important role in straw biomass depolymerization. Since fungi have physiological limitations to survive and grow in high-temperature environments, we set out different pasteurization regimes (57 °C/6 h, 60 °C/2 h, and 68 °C/2 h) to evaluate the impact on the fungal community assembly. The fungal community profile generated by high-throughput sequencing showed shifts in community diversity and composition under different pasteurization regimes. Most of the recovered sequences belong to the Ascomycota phylum. Among 73 species detected, Mycothermus thermophilus, Talaromyces thermophilus, and Thermomyces lanuginosus were the most abundant. In the current study, we outlined that pasteurization regimes can reshape the fungal community in compost which can potentially impact the A. bisporus development.

双孢蘑菇栽培基于通过精细堆肥过程制备的选择性基质，其中嗜热和/或耐热真菌可能在秸秆生物质解聚中发挥重要作用。由于真菌在高温环境中生存和生长具有生理限制，我们制定了不同的巴氏杀菌方案（57 °C/6 h、60 °C/2 h 和 68 °C/2 h）来评估对高温环境的影响。真菌社区集会。高通量测序产生的真菌群落概况显示在不同的巴氏杀菌制度下群落多样性和组成发生了变化。大多数恢复的序列属于子囊菌门。在检测到的 73 个物种中，Mycothermus thermophilus、Talaromyces thermophilus和Thermomyces lanuginosus是最丰富的。在当前的研究中，我们概述了巴氏杀菌制度可以重塑堆肥中的真菌群落，这可能会影响A。双孢子虫的发展。