Amyloid aggregation and neurofibrillary pathology is the characteristic feature of Alzheimer's disease (AD). Streptozotocin (STZ) is a glucosamine nitrosourea compound that is toxic to the cells, impairs insulin signaling in the brain. STZ induces DNA damage and oxidative stress, which leads to cognitive impairments. In this experimental study, STZ treatment induces Alzheimer's pathology in mouse neuroblastoma (N2A) cells. The study also explored the protection of cellular damage by pre-treatment with test drugs Apple cider vinegar (ACV), chrysin and rivastigmine. This study had been concentrated mainly on the cellular mechanism of neuromodulation and anti –oxidant potency of test drugs to attenuate cellular toxicity induced by STZ treatment. The 100 µM concentration of STZ was used for treatment in N2A cells for 24 h and 48 h and multiple studies were performed. The STZ causes tau phosphorylation, amyloid aggregation and increased acetyl cholinesterase (AChE) activity. Along with STZ at a concentration of 100 µM, the cells were pre-treated with ACV, chrysin and rivastigmine at a concentration of 2, 10, 50 µM each. The results show that 2 µM test drug concentration presented considerable protection, against STZ generated neurocytotoxicity via. restoration of anti-oxidant enzymes, MDA and AChE level, compared to the other two concentrations.

中文翻译：

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通过细胞研究发现多酚的治疗潜力-通过下调AChE活性对苹果醋（ACV）和Chrysin对抗阿尔茨海默氏病样病理的体外研究

淀粉样蛋白聚集和神经原纤维病理是阿尔茨海默氏病（AD）的特征。链脲佐菌素（STZ）是一种氨基葡萄糖亚硝基脲化合物，对细胞有毒，可损害大脑中的胰岛素信号。STZ诱导DNA损伤和氧化应激，从而导致认知障碍。在这项实验研究中，STZ治疗在小鼠神经母细胞瘤（N2A）细胞中诱导了阿尔茨海默氏病的病理。该研究还探索了通过用测试药物苹果酒醋（ACV），绿素和利凡斯的明进行预处理来保护细胞损伤的方法。这项研究主要集中在受试药物减轻STZ处理诱导的细胞毒性的神经调节和抗氧化能力的细胞机制上。将浓度为100 µM的STZ在N2A细胞中处理24小时和48小时，并进行了多次研究。STZ引起tau磷酸化，淀粉样蛋白聚集和乙酰胆碱酯酶（AChE）活性增加。将细胞与浓度为100 µM的STZ一起，分别用浓度分别为2、10、50 µM的ACV，菊花素和卡巴拉汀进行预处理。结果表明，2 µM的受试药物浓度可有效抵抗STZ产生的神经细胞毒性。与其他两个浓度相比，抗氧化酶，MDA和AChE含量得以恢复。分别为2、10、50 µM的金黄色葡萄球菌素和卡巴拉汀。结果表明，2 µM的受试药物浓度可有效抵抗STZ产生的神经细胞毒性。与其他两个浓度相比，抗氧化酶，MDA和AChE含量得以恢复。分别为2、10、50 µM的金黄色葡萄球菌素和卡巴拉汀。结果表明，2 µM的受试药物浓度可有效抵抗STZ产生的神经细胞毒性。与其他两个浓度相比，抗氧化酶，MDA和AChE含量得以恢复。