Background: Infrared (IR) spectroscopy is a well-established technique for the structural elucidation of simple as well complex molecules. It has wide applications in the qualitative as well as quantitative determination of proteins in different samples. It provides a clear picture of the primary, secondary, or tertiary structure of a protein. Infrared radiations are used to assess different vibrational modes arise from variations in the structural components of a protein.

Methods: Various research reports were collected from search engines like Sciencedirect, Pubmed, Researchgate, and Google Scholar. They were further studied thoroughly and important findings/ data were compiled and represented with tables and figures. The procured data, which includes bandwidth, frequency and intensity, have been employed to elucidate the structure of a protein.

Results: It was found from various reports that Fourier transforms infrared spectroscopy (FT-IR) has widely been utilized to predict the secondary structure of the protein in the past few years. FTIR has the ability to trace out various structural modifications in the protein structure that originate due to interactions with other materials. It is also evident that it can be utilized to quantify the proteins in a variety of samples.

Conclusion: The present review describes the basic principle and the instrumentation of IR spectroscopy and its advancements. Beyond this, various applications of this technique in determining protein structure and quantification in different materials such as food stuffs, biotechnological products and biological fluids have also been summarized.

背景：红外（IR）光谱技术是一种用于阐明简单以及复杂分子的结构的成熟技术。它在不同样品中蛋白质的定性和定量测定中具有广泛的应用。它提供了蛋白质的一级，二级或三级结构的清晰图片。红外辐射用于评估由于蛋白质结构成分的变化而产生的不同振动模式。

方法：从Sciencedirect，Pubmed，Researchgate和Google Scholar等搜索引擎收集了各种研究报告。对它们进行了深入的研究，并汇编了重要的发现/数据并用表格和数字表示。获得的数据（包括带宽，频率和强度）已用于阐明蛋白质的结构。

结果：从各种报告中发现，傅立叶变换红外光谱（FT-IR）在过去几年中已广泛用于预测蛋白质的二级结构。FTIR具有追溯蛋白质结构中由于与其他物质相互作用而产生的各种结构修饰的能力。同样明显的是，它可以用于定量各种样品中的蛋白质。

结论：本综述描述了红外光谱的基本原理和仪器及其进展。除此之外，还总结了该技术在确定蛋白质结构和定量分析不同材料（例如食品，生物技术产品和生物流体）中的各种应用。