Biochemical investigation of bioactive compounds from the marine demospongiae Clathria (Thalysias) vulpina (Lamarck, 1814) (family Microcionidae) resulted in the isolation of two previously undescribed 22-membered macrocyclic lactone derivatives clathrolide A and B from its organic extract. Structural elucidation of the compounds were carried out using spectroscopic analysis. Clathrolide A exhibited greater antihypertensive (IC₅₀ 0.44 ± 0.02 mM), anti-inflammatory (IC₅₀ 0.74 ± 0.04 mM), anti-hyperglycemic (IC₅₀ 0.85 ± 0.01 mM), and antioxidant (IC₅₀ 0.70 ± 0.03 mM) activities compared to clathrolide B. Among various bioactivities, such as antihypertensive, anti-inflammatory, anti-hyperglycemic, and antioxidant properties, angiotensin converting enzyme attenuation potential of clathrolide A was found to be significantly greater, and comparable with the standard antihypertensive drug, captopril (IC₅₀ 0.36 ± 0.03 mM). Higher electronic properties (topological polar surface area 133.52) along with comparatively lesser docking score and binding energy of clathrolide A with the aminoacyl residues of angiotensin-I converting enzyme (−12.38 and −12.91 kcal/mol, respectively) recognized its prospective inhibitory property against the enzyme catalyzing the rate-limiting step to form the vasoconstrictor angiotensin-II.

生化研究了来自海洋脱皮鼠笼草（Thalysias）vulpina（Lamarck，1814）（Microcionidae家族）的生物活性化合物，从其有机提取物中分离出了两个先前未描述的22元大环内酯衍生物笼草内酯A和B。使用光谱分析进行化合物的结构阐明。Clathrolide A表现出更高的抗高血压（IC ₅₀ 0.44±0.02 mM），抗炎（IC ₅₀ 0.74±0.04 mM），抗血糖（IC ₅₀ 0.85±0.01 mM）和抗氧化剂（IC ₅₀相较于笼草肽B，其活性为0.70±0.03 mM）。在多种生物活性中，例如抗高血压，抗炎，降血糖和抗氧化特性，发现笼草肽A的血管紧张素转化酶的衰减潜能显着增强，并且与笼草B相当。标准降压药卡托普利（IC ₅₀ 0.36±0.03 mM）。较高的电子性能（拓扑极性表面积133.52）以及相对较小的笼形化合物A与血管紧张素I转化酶的氨酰基残基的对接分数和结合能（分别为-12.38和-12.91 kcal / mol）认识到其对该酶催化限速步骤以形成血管收缩剂血管紧张素-II。