Objective The objective of this study is to evaluate the feasibility and tolerability of new bacteriological samples to diagnose tuberculosis (TB) in HIV-infected children. Method and patients HIV1-infected children with suspicion of TB in Universitary Hospital Sourô Sanon (Burkina Faso) were included in a prospective cohort study. Children underwent three gastric aspirates (GA) if aged <4 years; two GA, one string test (ST) if aged 4–9 years and three sputum, one ST if aged 10–13 years. All children underwent one nasopharyngeal aspirate (NPA) and one stool sample. To assess feasibility and tolerability of procedures, adverse events were identified and pain was rated on different scales. Samples were tested by microscopy, culture, GeneXpert® (Xpert®). Results Sixty-three patients were included. Mean age was 8.92 years, 52.38% were females. Ninety-five GA, 67 sputum, 62 NPA, 60 stool and 55 ST had been performed. During sampling, the main adverse events were cough at 68/95 GA and 48/62 NPA; sneeze at 50/95 GA and 38/62 NPA and vomiting at 4/55 ST. On the behavioral scale, the average pain score during collection was 6.38/10 for GA; 7.70/10 for NPA and 1.03/10 for ST. Of the 31 cases of TB, bacteriological confirmation was made in 12 patients. Conclusion ST, stool is well-tolerated alternatives specimens for diagnosing TB in children. NPA has a poor feasibility and tolerability in children.

中文翻译：

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细菌学采集工具在 HIV 感染儿童结核病诊断中的贡献和可接受性

目的 本研究的目的是评估新的细菌样本在 HIV 感染儿童中诊断结核病 (TB) 的可行性和耐受性。方法和患者 Sourô Sanon（布基纳法索）大学医院疑似结核病的 HIV1 感染儿童被纳入一项前瞻性队列研究。年龄小于 4 岁的儿童接受 3 次胃抽吸 (GA)；两次 GA，如果 4-9 岁，则进行一次字符串测试 (ST) 和 3 次痰，如果年龄在 10-13 岁，则进行一次 ST。所有儿童都接受了一份鼻咽抽吸物 (NPA) 和一份粪便样本。为了评估程序的可行性和耐受性，确定了不良事件并对疼痛进行了不同的等级评定。通过显微镜、培养、GeneXpert® (Xpert®) 对样品进行测试。结果共纳入63例患者。平均年龄为 8.92 岁，52.38% 为女性。95 次 GA、67 次痰、62 次 NPA、60 次粪便和 55 次 ST。采样期间，主要不良事件为 68/95 GA 和 48/62 NPA 的咳嗽；在 50/95 GA 和 38/62 NPA 打喷嚏，在 4/55 ST 呕吐。在行为量表上，GA 采集期间的平均疼痛评分为 6.38/10；NPA 为 7.70/10，ST 为 1.03/10。31例结核病患者中，12例进行了细菌学证实。结论 ST、粪便是诊断儿童结核病耐受性良好的替代标本。NPA在儿童中的可行性和耐受性较差。NPA 为 70/10，ST 为 1.03/10。31例结核病患者中，12例进行了细菌学证实。结论 ST、粪便是诊断儿童结核病耐受性良好的替代标本。NPA在儿童中的可行性和耐受性较差。NPA 为 70/10，ST 为 1.03/10。31例结核病患者中，12例进行了细菌学证实。结论 ST、粪便是诊断儿童结核病耐受性良好的替代标本。NPA在儿童中的可行性和耐受性较差。