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Generation and characterization of a tamoxifen-inducible Vsx1-CreERT2 line to target V2 interneurons in the mouse developing spinal cord
genesis ( IF 1.5 ) Pub Date : 2021-05-26 , DOI: 10.1002/dvg.23435
Charlotte Baudouin 1 , Barbara Pelosi 1 , Guillaume E Courtoy 2 , Younes Achouri 3 , Frédéric Clotman 1
Affiliation  

In the spinal cord, ventral interneurons regulate the activity of motor neurons, thereby controlling motor activities including locomotion. Interneurons arise during embryonic development from distinct progenitor domains orderly distributed along the dorso-ventral axis of the neural tube. The p2 progenitor domain generates at least five V2 interneuron populations. However, identification and characterization of all V2 populations remain currently incomplete and the mechanisms that control their development remain only partly understood. In this study, we report the generation of a Vsx1-CreERT2 BAC transgenic mouse line that drives CreERT2 recombinase expression mimicking endogenous Vsx1 expression pattern in the developing spinal cord. We showed that the Vsx1-CreERT2 transgene can mediate recombination in V2 precursors with a high efficacy and specificity. Lineage tracing demonstrated that all the V2 interneurons in the mouse developing spinal cord derive from cells expressing Vsx1. Finally, we confirmed that V2 precursors generate additional V2 populations that are not characterized yet. Thus, the Vsx1-CreERT2 line described here is a useful genetic tool for lineage tracing and for functional studies of the mouse spinal V2 interneurons.

中文翻译:

三苯氧胺诱导型 Vsx1-CreERT2 系的产生和表征,以靶向发育脊髓的小鼠 V2 中间神经元

在脊髓中,腹侧中间神经元调节运动神经元的活动,从而控制包括运动在内的运动活动。中间神经元在胚胎发育过程中从沿神经管背腹轴有序分布的不同祖域产生。p2 祖域产生至少五个 V2 中间神经元群体。然而,目前对所有 V2 种群的识别和表征仍然不完整,控制其发育的机制仍然只是部分了解。在这项研究中,我们报告了Vsx1-CreER T2 BAC 转基因小鼠品系的产生,该品系驱动 CreER T2重组酶表达模拟内源性Vsx1发育中脊髓的表达模式。我们表明,Vsx1-CreER T2转基因可以以高效和特异性介导 V2 前体中的重组。谱系追踪表明,小鼠脊髓发育中的所有 V2 中间神经元均来自表达Vsx1的细胞。最后,我们确认 V2 前体产生了尚未表征的额外 V2 种群。因此,这里描述的Vsx1-CreER T2线是一种有用的遗传工具,用于谱系追踪和小鼠脊柱 V2 中间神经元的功能研究。
更新日期:2021-05-26
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