ABSTRACT

The main purpose of the present study is to predict the optimal values of the ultrasound-extraction conditions by employing the response surface method based on Box–Behnken design from Moringa oleifera leaves. The impact of three independent variables, namely the extraction time (5,15, and 25 min), solid to the solvent ratio (60%, 70%, and 80%), and ultrasonic amplitude (20%, 60%, and 100%) on the extraction yield and total phenolic/flavonoid content were studied. Analysis of variance (ANOVA) was employed to estimate the significance of the independent parameters and the model. An extraction time of 15 min, an ultrasonic amplitude of 100%, and a solid to solvent ratio of 80% were found to be optimal values. The findings of this investigation revealed that at optimal independent factors, the values of the extraction yield, total flavonoids, and phenolics content were found to be 83.75 mgL⁻¹,50 mgL⁻¹, and 0.974 g, respectively. The obtained results indicated that BBD-RSM design could effectively be used in the optimization of the ultrasonic-assisted extraction process. To the best of our knowledge, this is the first study assessing the effect of US power, time of extraction, and solid to the solvent ratio on the extraction yield and phenolics content/flavonoids content of Moringa oleifera leaves cultivated in Iran.

摘要

本研究的主要目的是通过基于辣木Box-Behnken设计的响应面方法来预测超声提取条件的最佳值。树叶。三个独立变量的影响，分别是提取时间（5,15和25分钟），固体与溶剂的比例（60％，70％和80％）和超声振幅（20％，60％和100） ％）的提取率和总酚/类黄酮含量进行了研究。方差分析（ANOVA）用于估计独立参数和模型的重要性。发现15分钟的提取时间，100％的超声振幅和80％的固溶比是最佳值。该研究的结果显示，在最佳的独立因素，发现提取率，总黄酮，和酚含量的值是83.75 MGL ^-1，50 MGL ^-1和分别为0.974克。所得结果表明，BBD-RSM设计可以有效地用于超声辅助提取工艺的优化。据我们所知，这是第一项评估美国力量，提取时间和固液比对伊朗种植的辣木叶提取率和酚类含量/类黄酮含量影响的研究。