The aim of this study was to investigate the antioxidant activity of Weissella confusa CGMCC 19,308 and its influence on longevity and host defense against Salmonella Typhimurium of Caenorhabditis elegans. The CFCS (cell-free culture supernatant) of W. confusa CGMCC 19,308 possessed DPPH radicals, hydroxyl radicals, and superoxide anion scavenging activity. The lifespan of the C. elegans fed W. confusa CGMCC 19,308 was significantly (p < 0.001) longer than that of worms fed Escherichia coli OP50. Moreover, worms fed W. confusa CGMCC 19,308 were more resistant to oxidative stress induced by hydrogen peroxide and S. Typhimurium infection. RNA-seq analysis showed that the most significantly differentially expressed genes (DEGs) in C. elegans fed with W. confusa CGMCC 19,308 were mainly col genes (col-43, col-2, col-40, col-155, col-37), glutathione–S-transferase (GST)-related genes (gst-44, gst-9, gst-17, gst-18, gstk-2), cnc-9 (immune-related gene), and sod-5 (superoxide dismutase). These results indicated that cuticle collagen synthesis, immunity, and antioxidant defense (AOD) system of C. elegans were affected after being fed with W. confusa CGMCC 19,308 instead of E. coli OP50. Our study suggested W. confusa CGMCC 19,308 had the antioxidant activity and could prolong lifespan and enhance the host defense against S. Typhimurium of C. elegans. This study provided new evidences for the W. confusa CGMCC 19,308 as a potential probiotic candidate for anti-aging and anti-bacterial infection.

本研究的目的是研究Weissella confusa CGMCC 19,308 的抗氧化活性及其对秀丽隐杆线虫鼠伤寒沙门氏菌的寿命和宿主防御的影响。W. confusa CGMCC 19,308的 CFCS（无细胞培养上清液）具有 DPPH 自由基、羟基自由基和超氧阴离子清除活性。喂食W. confusa CGMCC 19,308的C. elegans的寿命显着 ( p  < 0.001) 长于喂食大肠杆菌OP50 的蠕虫。此外，蠕虫喂养W. confusaCGMCC 19,308 对过氧化氢和鼠伤寒沙门氏菌感染引起的氧化应激具有更强的抵抗力。RNA-seq 分析表明，在喂食W. confusa CGMCC 19,308 的秀丽隐杆线虫中最显着的差异表达基因 (DEGs) 主要是col基因（col-43、col-2、col-40、col-155、col-37 )、谷胱甘肽-S-转移酶 (GST) 相关基因（gst-44、gst-9、gst-17、gst-18、gstk-2）、cnc-9（免疫相关基因）和sod-5（超氧化物歧化酶）。这些结果表明，在喂食W. confusa CGMCC 19,308 而不是大肠杆菌OP50后，秀丽隐杆线虫的角质层胶原合成、免疫和抗氧化防御 (AOD) 系统受到影响。我们的研究表明W. confusa CGMCC 19,308 具有抗氧化活性，可以延长寿命并增强宿主对秀丽隐杆线虫鼠伤寒沙门氏菌的防御。该研究为W. confusa CGMCC 19,308 作为抗衰老和抗菌感染的潜在益生菌候选者提供了新的证据。